P5726

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 2

aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

Quality Level

form

aqueous solution (dark coloration may develop upon storage, which does not affect the activity)

shipped in

wet ice

storage temp.

2-8°C

Application

The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been optimized and tested for tyrosine protein phosphatases, acid and alkaline phosphatases. It has been tested on cell extracts from animal tissues including bovine liver and human placenta, and cytosolic extract of rabbit muscle.

Biochem/physiol Actions

This mixture contains individual components with specific inhibitory properties. Sodium orthovanadate inhibits a number of ATPases, protein tyrosine phosphatases, and other phosphate-transferring enzymes. Sodium molybdate inhibits acid and phosphoprotein phosphatases. Sodium tartrate inhibits acid phosphatases. Imidazole inhibits alkaline phosphatases.

Caution

As supplied, this product is stable for two years at the recommended 2-8°C storage temperature.

Preparation Note

This cocktail is supplied as a clear aqueous solution. It has been sterile filtered through a 0.2 μm membrane in bottles that have been aseptically filled. One mL will inhibit phosphatase activities found in the 100,000 x g supernatant from human placenta, bovine liver, rabbit muscle, A431, or Jurkat cell extracts at a protein concentration of approximately 5 mg/mL.

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Certificate of Analysis
Certificate of Origin
Colin S McCoin et al.
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Journal of Alzheimer's disease : JAD, 70(3), 861-875 (2019-07-16)
This study examined the effects of apolipoprotein E4 (APOE4), the most prevalent genetic risk factor for Alzheimer's disease (AD), on proteins involved in mitochondrial dynamics and autophagy, in the hippocampus of targeted replacement mice. Immunohistochemical measurements revealed that the levels...
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The Journal of biological chemistry, 291(48), 25247-25254 (2016-10-19)
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Articles
Information on Phosphatase Inhibitor Cocktail for researchers and students from MilliporeSigma. Phosphorylation is a reversible protein post-translational modification.
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