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P5747

Sigma-Aldrich

Monoclonal Anti-Phosphoserine antibody produced in mouse

clone PSR-45, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Monoclonal Anti-Phosphoserine, Phospho Ser, Phospho serine, Phospho−Ser, Phospho−serine

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

PSR-45, monoclonal

form

buffered aqueous solution

packaging

antibody small pack of 25 μL

concentration

~2 mg/mL

technique(s)

dot blot: suitable
indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA
microarray: suitable
western blot: 2.5-5.0 μg/mL using total rat brain extract.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
P6623P1869P3555
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

ascites fluid

clone

PSR-45, monoclonal

clone

PTR-8, monoclonal

clone

pT-154, monoclonal

clone

PTR-8, monoclonal

biological source

mouse

biological source

mouse

biological source

-

biological source

mouse

technique(s)

dot blot: suitable, microarray: suitable, indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA, western blot: 2.5-5.0 μg/mL using total rat brain extract.

technique(s)

dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1 μg/mL, microarray: suitable, western blot: 5-10 μg/mL using A431 cell extracts

technique(s)

direct ELISA: suitable, immunohistochemistry: suitable, microarray: suitable, western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF

technique(s)

indirect ELISA: 1:4,000, western blot: 1:50

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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Specificity

By ELISA and dot blot, the antibody reacts specifically with phosphorylated serine, both as free amino acid or conjugated to carriers such as BSA or KLH. No cross-reactivity is observed with non-phosphorylated serine, phosphothreonine, phosphotyrosine, AMP or ATP. This antibody has been used in immunoblotting for the localization of some phosphoserine-containing proteins. Certain proteins known to contain phosphorylated serine may not be recognized by this antibody due to steric hindrance of the recognition site.

Immunogen

phosphoserine conjugated to Keyhole Limpet Hemocyanin (KLH).

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
The antibody has been used for the detection of some phosphoserine containing proteins using immunoblotting and dot blotting.

Biochem/physiol Actions

Protein phosphorylation and dephosphorylation are basic signaling mechanisms that modify protein function in eukaryotic cells. Phosphorylation is a rare posttranslational event in normal tissues, however, the abundance of phosphorylated cellular proteins increases several folds following various activation processes. The main amino acids that are phosphorylated are tyrosine, serine, or threonine (pTyr/pSer/pThr), each having specific kinases that phosphorylate them and specific phosphatases that dephosphorylate them. Mono and polyclonal antibodies directed against phosphorylated residues were generated and found useful as analytical and preparative tools by enabling the identification, quantification, and immunoaffinity isolation of phosphorylated cellular proteins.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Related product

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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An integrated assessment of histopathological changes of the enteric neuromuscular compartment in experimental colitis
Ippolito C, et al.
Journal of Cellular and Molecular Medicine, 19(2), 485-500 (2015)
Matteo Fornai et al.
The Journal of pharmacology and experimental therapeutics, 356(2), 434-444 (2015-11-20)
Parkinson's disease is frequently associated with gastrointestinal symptoms, mostly represented by constipation and defecatory dysfunctions. This study examined the impact of central dopaminergic denervation, induced by injection of 6-hydroxydopamine (6-OHDA) into the medial forebrain bundle, on distal colonic excitatory cholinergic
Mechanisms of specificity in protein phosphorylation
Ubersax JA and Ferrell Jr JE
Nature Reviews in Molecular and Cell Biology, 8(7), 530-530 (2007)
Ling Lai et al.
Journal of pineal research, 45(4), 476-488 (2008-08-19)
Melatonin, via its MT1 receptor, but not the MT2 receptor, can modulate the transcriptional activity of various nuclear receptors - estrogen receptor alpha (ERalpha) and retinoic acid receptor alpha (RARalpha), but not ERbeta- in MCF-7, T47D, and ZR-75-1 human breast
Changes in expression of VE-cadherin and MMPs in endothelial cells: Implications for angiogenesis
Kiran MS, et al.
Vascular Cell, 3(1), 6-6 (2011)

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