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P5813

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone BP53-12, ascites fluid

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Synonym(s):
Anti-TRP53, Anti-Transformation-related protein 53
MDL number:
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BP53-12, monoclonal

mol wt

antigen 53 kDa

contains

15 mM sodium azide

species reactivity

human

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using methacarn-fixed, paraffin-embedded sections of human malignant tissue
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: suitable using cell lysates from human cell lines known to express high levels of the p53 protein

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)

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This Item
P6874P8999P6749
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

BP53-12, monoclonal

clone

DO-1, monoclonal

clone

DO-7, monoclonal

clone

PAb1801, monoclonal

mol wt

antigen 53 kDa

mol wt

antigen ~53 kDa

mol wt

antigen ~53 kDa

mol wt

antigen ~53 kDa

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

General description

Monoclonal Anti-p53 (mouse Ig2a isotype) is derived from the BP53-12 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human wild-type p53 protein. The tumor suppressor gene TP53 (gene) codes for a transcription factor. TP53 gene is mapped to human chromosome 17p13.1. The human wild-type p53 protein is a 393 amino acid nuclear phosphoprotein, present in the nucleus of all normal mammalian cells.

Immunogen

recombinant human wild-type p53 protein.

Application

Monoclonal Anti-p53 antibody produced in mouse has been used in:
  • western blotting
  • immunohistochemistry
  • immunocytochemistry
  • enzyme linked immunosorbent assay (ELISA)

Mouse monoclonal clone BP53-12 anti-p53 antibody is a useful tool suitable for the detection and quantitation of p53 applying various immunochemical techniques, especially immunohistochemistry using conventionally fixed and processed histological sections. The product is also useful for immunoprecipitation, ELISA and immunocytochemical staining of cultured cell lines.

Biochem/physiol Actions

The tumor suppressor gene TP53 (gene) gene is involved in the regulation of cell cycle, senescence and apoptosis upon oncogene activation, stress and DNA damage. Point mutations of the wild-type gene have been suggested to be a key event in the development of malignancy as the mutant protein acts as a dominant oncogene. Indeed, mutations of the p53 gene are the most common molecular changes identified in human cancer. They have been reported to be a frequent feature of breast, lung, colon, ovarian, brain, testicular and bladder cancers, melanoma, neurofibrosarcoma, and certain types of leukemia. The accumulation of high levels of p53 is a potential novel marker for malignancy. Monoclonal Anti-p53 recognizes a denaturation resistant epitope on the primate p53 nuclear protein (53 kDa). It does not react with other cellular proteins.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Identification and characterization of different subpopulations in a human lung adenocarcinoma cell line (A549)
Croce MV, et al.
Pathology Oncology Research, 5(3), 197-204 (1999)
Ewelina Wawryk-Gawda et al.
Protoplasma, 251(3), 525-533 (2013-09-18)
The p53 protein is an important factor of many intra- and extracellular processes. This protein regulates the repair of cellular DNA and induces apoptosis. It is also responsible for the regulation of the senescence and the cell entering the subsequent
A p53-like protein from a freshwater mollusc Lamellidens corrianus
Mohanty BP
Indian Journal of Biochemistry & Biophysics, 121-139 (2006)
How narrow-band and broad-band uvb irradiation influences the histomorphology evaluations of experimental animals? skin-a comparative study
Borowska K
Dermatology Online Journal, 8(3e), e5-e5 (2017)
P53 protein in proliferation, repair and apoptosis of cells
Wawryk GE, et al.
Protoplasma, 251(3), 525-533 (2014)

Articles

p53: DNA Damage Response and Cancer

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

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