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P5975

Amino-terminal Met-FLAG-BAP™ Fusion Protein

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P5975-.1MG

$386.00

$386.00

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About This Item

MDL number:
MFCD01632954
UNSPSC Code:
12352202
NACRES:
NA.32

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mol wt

~49 kDa

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

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This Item
P7457P7582SRP0594
Amino-terminal Met-FLAG-BAP™ Fusion Protein

Millipore

P5975

Amino-terminal Met-FLAG-BAP™ Fusion Protein

Carboxy-terminal FLAG-BAP™ Fusion Protein

Sigma-Aldrich

P7457

Carboxy-terminal FLAG-BAP™ Fusion Protein

Amino-terminal FLAG-BAP™ Fusion Protein

Sigma-Aldrich

P7582

Amino-terminal FLAG-BAP™ Fusion Protein

Mouse TDO2, His-Tag recombinant, expressed in E. coli

Sigma-Aldrich

SRP0594

Mouse TDO2, His-Tag

mol wt

~49 kDa

mol wt

~49 kDa

mol wt

~49 kDa

mol wt

49 kDa

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−70°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

Application

Amino-terminal Met-FLAG-BAP™ Fusion Protein is useful for assurance of the functional integrity of ANTI-FLAGÒ M2 and M5 monoclonal antibodies in immunological procedures such as Western blotting, ELISA, immunoprecipitation, fluorescence microscopy, light microscopy and FACS.
Learn more product details in our FLAG® application portal.

Biochem/physiol Actions

Amino-terminal Met-FLAG-BAP™ control protein is a 468 amino acid N-terminal Met-FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular weight of 49.4 kDa. The Amino-terminal Met-FLAG-BAP control protein migrates as a 45-55 kDa band by SDS-PAGE depending on the electrophoretic conditions.

Other Notes

Control protein

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLCK0200
099H9110
090K9083
039H9006
039H9004

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Protocols

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Related Content

Purificación de proteínas

Técnicas, reactivos y protocolos de purificación de proteínas para purificar proteínas recombinantes utilizando métodos como la cromatografía de intercambio iónico, de exclusión por tamaño y de afinidad.

Protein Purification

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Data Sheet - P5975

Instructions

EZview Red Protein A and ANTI-FLAG M2 Affinity Gels

EZviewTM Red Protein A and ANTI-FLAG® M2 Affinity Gels: Immunoprecipitation with Enhanced Visibility Affinity Beads - Technical Article - July 2001

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