Documents
P6001
p-Phenylenediamine
98% (GC)
Quality Level
vapor density
3.7 (vs air)
vapor pressure
1.08 mmHg ( 100 °C)
description
anti-fade reagent
assay
98% (GC)
bp
267 °C (lit.)
mp
138-143 °C (lit.)
SMILES string
Nc1ccc(N)cc1
InChI
1S/C6H8N2/c7-5-1-2-6(8)4-3-5/h1-4H,7-8H2
InChI key
CBCKQZAAMUWICA-UHFFFAOYSA-N
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Application
- to evaluate its sensitization potential in vitro in langerhans-like dendritic cells and autologous T cells
- to treat the dorsal sides of the ears of mice, to study its systemic immunological effects
- in glycerol to coverslip coronal sections in the attempt to count Aβ plaques
Anti-fade reagent is added to the mounting medium in fluorescence microscopy to retard photobleaching of fluorescein and other fluorescent dyes.
Packaging
Biochem/physiol Actions
Caution
Signal Word
Danger
Hazard Statements
Precautionary Statements
Personal Protective Equipment
RIDADR
UN 1673 6.1 / PGIII
WGK Germany
WGK 3
Flash Point(F)
230.0 °F - closed cup
Flash Point(C)
110 °C - closed cup
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the solubility of p-Phenylediamine, Product P6001?
This product is soluble in ethanol at a concentration of 50 mg/mL, with heating. A clear red to brown solution is observed.
For what applications is p-Phenylediamine, Product P6001, used?
This is an antifade reagent that is used as a fluorescent stabilizer. Do not confuse the p-phenylenediamine with o-phenylenediamine, which is used as a peroxidase substrate in ELISA procedures.
What is the working concentration for p-Phenylediamine, Product P6001, when used as an antifade agent?
J. Immunological Methods, 43, 349 (1981) describes a solution that will reduce the fading of fluorescent materials. The recipe for this solution is: "Add 10 mL of phosphate-buffered saline (0.01 M PO4, pH 7.4 in 0.15 M NaCl) containing 100 mg of p-phenylenediamine to 90 ml of glycerol. The final pH should be adjusted to approximately 8.0 with 0.5 M carbonate-bicarbonate buffer (pH 9.0)."
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