We have no information on using this product for oxidase test. Product No. T3134, N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride is commonly used to perform the oxidase test for the colorimetric identification of pathogenic and non-pathogenic bacteria.
P6001
p-Phenylenediamine
98% (GC)
Synonym(s):
1,4-Benzenediamine, 1,4-Diaminobenzene, 1,4-Phenylenediamine
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About This Item
Linear Formula:
C6H4(NH2)2
CAS Number:
Molecular Weight:
108.14
Beilstein/REAXYS Number:
742029
EC Number:
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
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vapor density
3.7 (vs air)
vapor pressure
1.08 mmHg ( 100 °C)
description
anti-fade reagent
assay
98% (GC)
bp
267 °C (lit.)
mp
138-143 °C (lit.)
SMILES string
Nc1ccc(N)cc1
InChI
1S/C6H8N2/c7-5-1-2-6(8)4-3-5/h1-4H,7-8H2
InChI key
CBCKQZAAMUWICA-UHFFFAOYSA-N
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This Item | 78429 | 695106 | P23954 |
|---|---|---|---|
| assay 98% (GC) | assay ≥99.0% (GC/NT) | assay ≥99% | assay 99% |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| bp 267 °C (lit.) | bp 267 °C (lit.) | bp 267 °C (lit.) | bp 282-284 °C |
| mp 138-143 °C (lit.) | mp 138-143 °C (lit.) | mp 138-143 °C (lit.) | mp 64-66 °C |
| vapor density 3.7 (vs air) | vapor density 3.7 (vs air) | vapor density 3.7 (vs air) | vapor density 3.7 (vs air) |
| vapor pressure 1.08 mmHg ( 100 °C) | vapor pressure 1.08 mmHg ( 100 °C) | vapor pressure 1.08 mmHg ( 100 °C) | vapor pressure 0.62 mmHg ( 100 °C) |
Application
p-Phenylenediamine has been used:
Anti-fade reagent is added to the mounting medium in fluorescence microscopy to retard photobleaching of fluorescein and other fluorescent dyes.
- to evaluate its sensitization potential in vitro in langerhans-like dendritic cells and autologous T cells[1]
- to treat the dorsal sides of the ears of mice, to study its systemic immunological effects[2]
- in glycerol to coverslip coronal sections in the attempt to count Aβ plaques[3]
Anti-fade reagent is added to the mounting medium in fluorescence microscopy to retard photobleaching of fluorescein and other fluorescent dyes.
Biochem/physiol Actions
p-Phenylenediamine causes allergic reactions with skin.[4] It is widely used in hair dyes. It eventually forms Bandrowski′s base, which is found to be the primary cause for allergy.[5] p-Phenylenediamine exposure results in dermatitis, urticaria and anaphylaxis.[6] It acts as an electron donor and is known to reduce cytochrome c.
Caution
Darkens in storage.
signalword
Danger
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Irrit. 2 - Skin Sens. 1 - STOT SE 1 Oral
target_organs
Kidney,Heart,Musculo-skeletal system
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
wgk_germany
WGK 3
flash_point_f
230.0 °F - closed cup
flash_point_c
110 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
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May I ask if I can use P- phenylenediamine for oxidase test
1 answer-
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What is the working concentration for p-Phenylediamine, Product P6001, when used as an antifade agent?
1 answer-
J. Immunological Methods, 43, 349 (1981) describes a solution that will reduce the fading of fluorescent materials. The recipe for this solution is: "Add 10 mL of phosphate-buffered saline (0.01 M PO4, pH 7.4 in 0.15 M NaCl) containing 100 mg of p-phenylenediamine to 90 ml of glycerol. The final pH should be adjusted to approximately 8.0 with 0.5 M carbonate-bicarbonate buffer (pH 9.0)".
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What is the solubility of p-Phenylediamine, Product P6001?
1 answer-
This product is soluble in ethanol at a concentration of 50 mg/mL, with heating. A clear red to brown solution is observed.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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For what applications is p-Phenylediamine, Product P6001, used?
1 answer-
This is an antifade reagent that is used as a fluorescent stabilizer. Do not confuse the p-phenylenediamine with o-phenylenediamine, which is used as a peroxidase substrate in ELISA procedures.
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