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Penicillinase from Bacillus cereus

Type II, lyophilized powder

Penicillin amido-β-lactam hydrolase, β-Lactamase II, Cephalosporinase, β-Lactamase I from Bacillus cereus
CAS Number:
Enzyme Commission number:
MDL number:


Type II


lyophilized powder


vial of ≥2.5 units (using cephaloridine)
vial of ≥300 units (using benzylpenicillin)

storage temp.


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Used in the production of penicillin.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

Unit Definition

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Physical form

Crude, lyophilized powder containing buffer and zinc salts, and stabilized with bovine serum albumin

Analysis Note

Typical vial content: approx. 10 mg total protein (biuret)

Certificate of Analysis

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Certificate of Origin

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