P6018

Penicillinase from Bacillus cereus

Type II, lyophilized powder

• Synonym(s): Penicillin amido-β-lactam hydrolase, β-Lactamase II, Cephalosporinase, β-Lactamase I from Bacillus cereus
• CAS Number: 9073-60-3
• Enzyme Commission number: 3.5.2.6 (BRENDA, IUBMB)
• MDL number: MFCD00131819

Properties

• type: Type II
• form: lyophilized powder
• packaging: vial of ≥2.5 units (using cephaloridine); vial of ≥300 units (using benzylpenicillin)
• storage temp.: 2-8°C

Description

Application

Used in the production of penicillin.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

Unit Definition

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Physical form

Crude, lyophilized powder containing buffer and zinc salts, and stabilized with bovine serum albumin

Analysis Note

Typical vial content: approx. 10 mg total protein (biuret)