P6282

Sigma-Aldrich

Poly-L-lysine hydrobromide

mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Synonym(s):
PLL HBr
CAS Number:
MDL number:
eCl@ss:
32160406
PubChem Substance ID:
NACRES:
NA.75

Quality Level

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

mol wt

70,000-150,000

packaging

pkg of 5 mg

application(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 50 mL/vial, clear, colorless

shipped in

ambient

storage temp.

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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Related Categories

Application

Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
Poly-L-lysine hydrobromide has been used to precoat glass bottom dishes used for in vitro cell culture.

Components

Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Other Notes

For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

Certificate of Analysis

Certificate of Origin

Oriane Mercati et al.
Biology open, 2(3), 324-334 (2013-03-23)
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Kasemeier KJC, et al.
The Journal of Neuroscience, 30(39), 13078-13088 (2010)
Rahul Munshi et al.
Frontiers in neuroscience, 12, 560-560 (2018-08-30)
Determining the role and necessity of specific neurons in a network calls for precisely timed, reversible removal of these neurons from the circuit via remotely triggered transient silencing. Previously, we have shown that alternating magnetic field mediated heating of magnetic...
Cornucopia of cultured Schwann cells: where is the best source in albino rats?
Mirajullah M, et al.
Journal of Ayub Medical College, Abbottabad : JAMC, 13(4), 3-4 (2012)
Mónica Díaz-Coránguez et al.
PloS one, 8(4), e60655-e60655 (2013-05-03)
Transit of human neural stem cells, ReNcell CX, through the blood brain barrier (BBB) was evaluated in an in vitro model of BBB and in nude mice. The BBB model was based on rat brain microvascular endothelial cells (RBMECs) cultured...
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Protocols
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