P6374

p53 human

recombinant, expressed in baculovirus infected Sf21 cells

• UNSPSC Code: 12352202
• NACRES: NA.25

Properties

• biological source: human
• Quality Level: 200
• recombinant: expressed in baculovirus infected Sf21 cells
• assay: ≥90% (SDS-PAGE)
• form: aqueous solution
• technique(s): dot blot: suitable
• UniProt accession no.: P04637
• application(s): genomic analysis
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... TP53(7157)

Description

General description

p53 protein is composed of an N-terminal domain with a transactivation domain and proline-rich region, a central core-DNA binding domain, and a C-terminal domain with tetramerization and regulatory domains.[1] The TP53 gene which encodes for the p53 protein is mapped on the human chromosome at 17p13.1.[2]

Application

Useful for the study of postranslational modification of p53, or protein-protein interaction studies.

p53 human has been used in dot blot.[3]

Biochem/physiol Actions

In addition, p53 was recently shown to induce differentiation of human embryonic stem cells. The effect is dependent on the DNA binding activity of p53. [4]

p53 gene is highly conserved and expressed in normal tissues. It is the most commonly mutated gene in human cancer and more then 500 gene mutations have been described in various types of malignancies, hematologic as well as solid tumors. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells. Thus, p53 plays a vital role in suppressing the development of cancer.

p53 protein acts as a transcription factor that participates in regulating positively or negatively the expression of several responsive genes. It plays a role in regulating angiogenesis, cellular senescence, autophagy, cell survival, differentiation, and oxidative stress. Mutations in the TP53 gene lead to Li Fraumeni cancer syndrome.[5]

Physical properties

Histidine-tagged, full length human p53

Physical form

Supplied as a solution in 20 mM Tris-HCl, pH 8.0, 20% glycerol, 100 mM KCl, 0.2 mM EDTA, and 1 mM DTT.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable