P6611

HIS-Select^® Nickel Affinity Gel

(1:1 suspension in a 20% ethanol solution)

• Synonym(s): Ni-NTA resin, nickel charged agarose
• NACRES: NA.56

Properties

• conjugate: magnetic beads
• Quality Level: 200
• form: (1:1 suspension in a 20% ethanol solution)
• feature: hydrophilic
• packaging: pkg of 1 mL; pkg of 100 mL; pkg of 25 mL; pkg of 5 mL; pkg of 500 mL
• concentration: 1.5-2.4 mL/mL (suspension in packed gel)
• technique(s): protein purification: suitable
• color: faint blue to very dark blue
• matrix: 6% Beaded Agarose
• capacity: >15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
• transition temp: flash point 32 °C (closed cup)
• storage temp.: 2-8°C

Description

General description

HIS-Select^® Nickel Affinity Gel is an immobilized metal ion affinity chromatography (IMAC) product, used for the purification of His-tagged proteins. While the unique, non-charged, hydrophilic linkage of the proprietary quadridentate NTA chelate group to the beaded agarose charged with nickel ensures high selectivity for small to medium scale His-tag protein purification, it also results in reduced non-specific binding of other proteins. HIS-Select Nickel Affinity Gel is selective for recombinant proteins with His-tags and exhibits low non-specific binding of other proteins. The selectivity can be modulated with the inclusion of imidazole during chromatography.

HIS-Select^® Nickel Affinity Gel is durable and can capture the recombinant proteins with histidine tags at a high flow rate. Recombinant proteins with histidine tags are bound using either native or denaturing conditions.

Application

HIS-Select^® Nickel Affinity Gel has been used in the purification of recombinant proteins like EF-hand calcium-binding protein (S100A14), LIM homeobox transcription factor 1 alpha protein, sigma-1 receptor as well as harpin, stable protein 1 (SP1), and BCR-ABL fusion protein.

Packaging

1, 5, 25, 100, 500 mL

Features and Benefits

• High selectivity for higher purity.
• Unique non-charged hydrophilic linkage reduces non-specific binding.
• Binding capacity for histidine-tagged protein is greater than 15 mg/mL.
• Binding under denaturing or non-denaturing conditions.
• One-step purification.
• Minimizes unwanted ionic interactions.
• Minimal nickel leaching.
• Bead size: 45-165 μm.

Linkage

It is also available with the EZview™ technology (Product Code E3528).

Physical form

1:1 suspension in a 20% ethanol solution

Storage and Stability

HIS-Select Nickel Affinity Gel is stable for at least one year when stored properly. The HIS-Select Nickel Affinity Gel should be cleaned after each use and an antimicrobial agent such as 20% ethanol should be added to the storage buffer.

Legal Information

HIS-Select is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P243
• Hazard Classifications: Flam. Liq. 3
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 3
• Flash Point(F): 89.6 °F - closed cup
• Flash Point(C): 32 °C - closed cup