Solutions prepared in water at 1 mg/mL may be stored in aliquots at -20°C for at least 6 months. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/076/p6621dat.pdf
P6621
Phospholipase C from Bacillus cereus
≥200 units/mg protein
Synonym(s):
Lecithinase C, Lipophosphodiesterase I, PC-PLC, Phosphatidylcholine cholinephosphohydrolase
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250 UNITS
$562.00
$562.00
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54
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lyophilized powder
Quality Level
specific activity
≥200 units/mg protein
mol wt
23-27 kDa by SDS-PAGE
greener alternative product characteristics
Waste Prevention
Design for Energy Efficiency
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solubility
H2O: soluble 1.0 mg/mL, faintly hazy to hazy
greener alternative category
storage temp.
−20°C
Gene Information
Bacillus cereus ATCC 10987 ... plC(2749087)
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Related Categories
1 of 4
This Item | P4039 | P7633 | 525186 |
|---|---|---|---|
| Gene Information Bacillus cereus ATCC 10987 ... plC(2749087) | Gene Information Clostridium perfringens str. 13 ... plc(988262) | Gene Information Clostridium perfringens str. 13 ... plc(988262) | Gene Information - |
| specific activity ≥200 units/mg protein | specific activity ≥150 units/mg protein | specific activity 10-50 units/mg protein | specific activity ≥50 units/mg dry wt. |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form solid |
| mol wt 23-27 kDa by SDS-PAGE | mol wt - | mol wt - | mol wt - |
| solubility H2O: soluble 1.0 mg/mL, faintly hazy to hazy | solubility - | solubility - | solubility aqueous buffer: soluble, water: soluble |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
General description
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in biodiesel research. For more information see the article in biofiles.
Application
Phospholipase C (PLC) is used to study adrenoceptor-mediated transmembrane signaling[1]. It is used to degrade inositol-containing phospholipids[2]. Phospholipase C from Bacillus, product P6621, has been used to study how flhF affects the export of PLC and other secretory virulence factors[3].
The enzyme from Sigma has been used in the digestion of phosphatidylserine fraction of Schistosoma mansoni adult worms.[4]
Biochem/physiol Actions
Hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
Phospholipase C is induced by thrombin and platelet-activating factor, forming 1,2-diacylglycerol and phosphatidic acid[2]. PLC hydrolyzes the phosphate bond on phosphatidylcholine and other glycerophospholipids yielding diacylglycerol; this enzyme will also hydrolyze the phosphate bonds of sphingomyelin, cardiolipin, choline plasmalogen and ceramide phospholipids.
Physical form
Lyophilized powder containing approx. 10% protein. Remainder; trehalose, zinc sulfate, and potassium phosphate
Other Notes
One unit will liberate 1.0 μmole of water soluble organic phosphorus from L-α-phosphatidylcholine per min at pH 7.3 at 37 °C.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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W Siess et al.
The Journal of biological chemistry, 259(13), 8286-8292 (1984-07-10)
The present study compares the molecular mechanism by which thrombin, platelet-activating factor, and epinephrine induce platelet activation. Thrombin and platelet-activating factor induce an initial activation of phospholipase C, as measured by formation of 1,2-diacylglycerol and phosphatidic acid, during platelet shape
Zollner, H.
Handbook of Enzyme Inhibitors, 387-387 (1993)
Clarisse Techer et al.
Food microbiology, 86, 103317-103317 (2019-11-11)
The famous French dessert "ile flottante" consists of a sweet egg white foam floating on a vanilla custard cream, which contains highly nutritive raw materials, including milk, sugar and egg. Spoilage issues are therefore a key concern for the manufacturers.
T Das et al.
The Journal of biological chemistry, 268(36), 27398-27405 (1993-12-25)
Our studies on the alpha 1-adrenoceptor signaling have demonstrated that the Gh family is a signal mediator. We report here that a 69-kDa phospholipase C (PLC) is the effector in this signal pathway. The enzyme was isolated by dissociating a
Sara Salvetti et al.
Microbiology (Reading, England), 153(Pt 8), 2541-2552 (2007-07-31)
Flagellar arrangement is a highly conserved feature within bacterial species. However, only a few genes regulating cell flagellation have been described in polar flagellate bacteria. This report demonstrates that the arrangement of flagella in the peritrichous flagellate Bacillus cereus is
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The recommended storage for this product is at -20°C in working aliquots.
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