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P6662

o-Phenylenediamine dihydrochloride

tablet, 1 mg substrate per tablet

Synonym(s):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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50 TABS

$218.00

100 TABS

$388.00

$218.00

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About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
615-28-1
Molecular Weight:
181.06
UNSPSC Code:
12352204
NACRES:
NA.83
PubChem Substance ID:
24898787
MDL number:
MFCD00012966
Beilstein/REAXYS Number:
3912045

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Product Name

o-Phenylenediamine dihydrochloride, tablet, 1 mg substrate per tablet

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

form

tablet

mp

258 °C (dec.) (lit.)

solubility

water: 1 tablet/10 mL, clear, colorless

storage temp.

2-8°C

Quality Level

200

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o-Phenylenediamine dihydrochloride tablet, 1 mg substrate per tablet

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o-Phenylenediamine dihydrochloride

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P8806

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 20 mg substrate per tablet

Sigma-Aldrich

P7288

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 15 mg substrate per tablet

Sigma-Aldrich

P4664

o-Phenylenediamine dihydrochloride

solubility

water: 1 tablet/10 mL, clear, colorless

solubility

-

solubility

-

solubility

water: 1 tablet/10 mL, clear, colorless

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

tablet

form

tablet

form

tablet

form

tablet

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

signalword

Warning

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Muta. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCD7006
SLCD4139
SLCF1731
SLBN6760V
SLBV4901

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