MilliporeSigma
All Photos(4)

P6757

Sigma-Aldrich

PIPES

≥99% (titration)

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
1,4-Piperazinediethanesulfonic acid, Piperazine-1,4-bis(2-ethanesulfonic acid), Piperazine-N,N′-bis(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O6S2
CAS Number:
Molecular Weight:
302.37
Beilstein:
817713
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

Assay

≥99% (titration)

form

crystalline powder

useful pH range

6.1-7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

Inchi Key

IHPYMWDTONKSCO-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
P8203P185180635
PIPES ≥99% (titration)

Sigma-Aldrich

P6757

PIPES

-
PIPES BioXtra, ≥99% (titration)

Sigma-Aldrich

P8203

PIPES

Premium Grade
PIPES BioPerformance Certified, suitable for cell culture

Sigma-Aldrich

P1851

PIPES

Premium Grade
PIPES BioXtra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

80635

PIPES

Premium Grade
form

crystalline powder

form

powder

form

crystalline powder

form

powder

mp

>300 °C (lit.)

mp

>300 °C (lit.)

mp

>300 °C (lit.)

mp

>300 °C (lit.)

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless

solubility

1 M NaOH: 0.5 M at 20 °C, clear, colorless

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless, soluble

solubility

0.1 M NaOH: 0.1 M at 20 °C, clear, colorless

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

100

General description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Application

Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative.,
Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater.
Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer.

Linkage

Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851) as well as several different salts for convenience in buffer preparation, and PIPES BioXtra (Product No. P8203) which is tested for the presence of trace metals.

Preparation Note

Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 3

1 of 3

ACES ≥99.0% (titration)

Sigma-Aldrich

A9758

ACES

Chromeazurol S Dye content 45 %

Sigma-Aldrich

199532

Chromeazurol S

MOPSO ≥99% (titration)

Sigma-Aldrich

M8389

MOPSO

The role of the buffer in the fixation of biological specimens for transmission and scanning electron microscopy
Schiff, R.I. and Gennaro, J.F., Jr.
Scanning, 2, 135-135 (1979)
K Yamamoto et al.
Histochemistry, 77(3), 339-351 (1983-01-01)
Effects of NaOH-PIPES buffer used as a vehicle for aldehyde fixative on alkaline phosphatase (ALPase) activity demonstrated cyto- and biochemically were compared with those of routinely used cacodylate buffer. The reaction products showing ALPase activity demonstrated ultracytochemically were confined to
A R Hardham
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 33(2), 110-118 (1985-02-01)
The study of the surface properties of zoospores and cysts of the fungus Phytophthora cinnamomi required a fixation regime that would preserve the cells adequately and not interfere with binding and detection of probes on the cell surface. When they
Use of PIPES buffer in the fixation of plant cells for electron microscopy
Salema, R. and Brandao, I.
Journal of Submicroscopic Cytology, 9, 79-79 (1973)
Buffers for enzymes.
J S Blanchard
Methods in enzymology, 104, 404-414 (1984-01-01)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service