P6757

Sigma-Aldrich

PIPES

≥99% (titration)

Synonym(s):
Piperazine-N,N′-bis(2-ethanesulfonic acid), Piperazine-1,4-bis(2-ethanesulfonic acid), 1,4-Piperazinediethanesulfonic acid
Empirical Formula (Hill Notation):
C8H18N2O6S2
CAS Number:
Molecular Weight:
302.37
Beilstein/REAXYS Number:
817713
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

assay

≥99% (titration)

form

crystalline powder

useful pH range

6.1 - 7.5

pKa (25 °C)

6.8

mp

>300 °C (lit.)

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless

Featured Industry

Diagnostic Assay Manufacturing

SMILES string

OS(=O)(=O)CCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O6S2/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16/h1-8H2,(H,11,12,13)(H,14,15,16)

InChI key

IHPYMWDTONKSCO-UHFFFAOYSA-N

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General description

PIPES is a member of the ethanesulfonic acid buffer series, first introduced by Good et al., developed to meet certain criteria: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and easily synthesized. Since its pKa at 37 °C is near physiological pH, PIPES has applications in cell culture work.

Application

Glutaraldehyde fixation of plant and animal tissue samples can cause loss of lipid, leading to apparent morphological changes. Lipid loss and artifacts were minimized when PIPES was used to buffer the glutaraldehyde fixative.,
Alkaline phosphatase activity is lost selectively from certain rat hepatocyte organelles when fixed for ultracytochemistry with cacodylate-buffered glutaraldehyde. When PIPES was used as buffer, retention of activity was 60% greater.
Fixation of fungal zoospores for fluorescence microscopy and electron microscopy was optimal with a combination of glutaraldehyde and formaldehyde in PIPES buffer.

Packaging

25, 100, 500 g in poly bottle
25 kg in poly drum
1 kg in poly bottle

Linkage

Sigma-Aldrich offers BioPerformance Certified cell culture-tested PIPES (Product No. P1851) as well as several different salts for convenience in buffer preparation, and PIPES BioXtra (Product No. P8203) which is tested for the presence of trace metals.

Preparation Note

Buffers can be prepared by adding a solution of base to PIPES free acid to titrate to the appropriate pH, or by mixing equimolar solutions of the monosodium salt and the disodium salt to titrate to the appropriate pH.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

K Yamamoto et al.
Histochemistry, 77(3), 339-351 (1983-01-01)
Effects of NaOH-PIPES buffer used as a vehicle for aldehyde fixative on alkaline phosphatase (ALPase) activity demonstrated cyto- and biochemically were compared with those of routinely used cacodylate buffer. The reaction products showing ALPase activity demonstrated ultracytochemically were confined to...
A R Hardham
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 33(2), 110-118 (1985-02-01)
The study of the surface properties of zoospores and cysts of the fungus Phytophthora cinnamomi required a fixation regime that would preserve the cells adequately and not interfere with binding and detection of probes on the cell surface. When they...
Use of PIPES buffer in the fixation of plant cells for electron microscopy
Salema, R. and Brandao, I.
Journal of Submicroscopic Cytology, 9, 79-79 (1973)
The role of the buffer in the fixation of biological specimens for transmission and scanning electron microscopy
Schiff, R.I. and Gennaro, J.F., Jr.
Scanning, 2, 135-135 (1979)
Buffers for enzymes.
J S Blanchard
Methods in enzymology, 104, 404-414 (1984-01-01)

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