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Anti-p53 antibody, Mouse monoclonal

clone DO-1, purified from hybridoma cell culture

Anti-P53, Anti-LFS1, Anti-BCC7, Anti-BMFS5, Anti-TRP53

Quality Level

biological source




antibody form

purified immunoglobulin

antibody product type

primary antibodies


DO-1, monoclonal


buffered aqueous solution

mol wt

antigen ~53 kDa

species reactivity



~2 mg/mL


flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 0.2-0.4 μg/mL using total cell extract of A431 cells



UniProt accession no.

shipped in

dry ice

storage temp.


Gene Information

human ... TP53(7157)

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General description

Monoclonal Anti-p53 (mouse IgG2a isotype) is derived from hybridoma DO-1 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on human chromosome 17p. The gene codes for a tumor suppressor protein that is expressed in normal tissues.


Mouse anti-(p53) antibody reacts specifically with (p53) if human.


recombinant human wild type p53. The epitope resides between amino acids 20-25 of human p53.


Anti-p53 antibody, Mouse monoclonal has been used in:
  • semi-dry western blotting
  • immunoblotting
  • immunofluorescence
  • enzyme-linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunohistochemistry
  • immunoprecipitation
  • flow cytometry
  • chromatin immunoprecipitation (ChIP) assay

Biochem/physiol Actions

The p53 tumor suppressor protein is important in the cellular response to DNA damage and other genomic aberrations. Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21awf1. Wild-type p53 is shown to be a sequence-specific transcription factor, directly interacting with various cellular and viral proteins. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells.p53 has a pivotal role in suppressing the development of cancer, cell cycle regulation and modulation of CAK kinase activity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Quotes and Ordering

Knockdown of hnRNPK leads to increased DNA damage after irradiation and reduces survival of tumor cells
Wiesmann N, et al.
Carcinogenesis, 38(3), 321-328 (2017)
Mikael S Lindström et al.
PloS one, 5(3), e9578-e9578 (2010-03-12)
Disruption of the nucleolus often leads to activation of the p53 tumor suppressor pathway through inhibition of MDM2 that is mediated by a limited set of ribosomal proteins including RPL11 and RPL5. The effects of ribosomal protein loss in cultured
Maria Yusenko et al.
Scientific reports, 8(1), 13159-13159 (2018-09-05)
The transcription factor MYB plays key roles in hematopoietic cells and has been implicated the development of leukemia. MYB has therefore emerged as an attractive target for drug development. Recent work has suggested that targeting MYB by small-molecule inhibitors is
B A Bonsing et al.
Cytometry, 28(1), 11-24 (1997-05-01)
p53 immunostaining of histological sections shows inter- and intratumor variability in distribution and staining intensity which are usually scored semiquantitatively. In order to investigate the variation in p53 expression more accurately and its possible relation to other cellular parameters (e.g.
E Schneider et al.
Oncogene, 17(21), 2733-2741 (1998-12-05)
The growth suppressor p53 is an important key element which controls cell cycle progression in response to cellular stress like DNA damage. Its ability to act as transcriptional activator or repressor links transcription and cell cycle control. Several target genes


p53: DNA Damage Response and Cancer

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

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