P6874

Anti-p53 antibody, Mouse monoclonal

clone DO-1, purified from hybridoma cell culture

• Synonym(s): Anti-BCC7, Anti-BMFS5, Anti-LFS1, Anti-P53, Anti-TRP53
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: DO-1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~53 kDa
• species reactivity: human
• concentration: ~2 mg/mL
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.2-0.4 μg/mL using total cell extract of A431 cells
• isotype: IgG2a
• UniProt accession no.: P04637
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... TP53(7157)

Description

General description

Monoclonal Anti-p53 (mouse IgG2a isotype) is derived from hybridoma DO-1 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on human chromosome 17p. The gene codes for a tumor suppressor protein that is expressed in normal tissues.

Specificity

Mouse anti-(p53) antibody reacts specifically with (p53) if human.

Immunogen

recombinant human wild type p53. The epitope resides between amino acids 20-25 of human p53.

Application

Anti-p53 antibody, Mouse monoclonal has been used in:

• semi-dry western blotting
• immunoblotting
• immunofluorescence
• enzyme-linked immunosorbent assay (ELISA)
• immunocytochemistry
• immunohistochemistry
• immunoprecipitation
• flow cytometry
• chromatin immunoprecipitation (ChIP) assay

Biochem/physiol Actions

The p53 tumor suppressor protein is important in the cellular response to DNA damage and other genomic aberrations. Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21^awf1. Wild-type p53 is shown to be a sequence-specific transcription factor, directly interacting with various cellular and viral proteins. Intact p53 function is essential for the maintenance of the non-tumorogenic phenotype of cells.p53 has a pivotal role in suppressing the development of cancer, cell cycle regulation and modulation of CAK kinase activity.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves