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MilliporeSigma

P6912

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

peroxidase substrate, chromogenic, tablet

Synonym(s):

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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50 TABS
$243.00
100 TABS
$411.00

$243.00

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About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
615-28-1
Molecular Weight:
181.06
Beilstein/REAXYS Number:
3912045
MDL number:
MFCD00012966
UNSPSC Code:
12352204
PubChem Substance ID:
24898805
NACRES:
NA.83

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Product Name

o-Phenylenediamine dihydrochloride, tablet, 5 mg substrate per tablet

Quality Level

300

form

tablet

mp

258 °C (dec.) (lit.)

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet

Sigma-Aldrich

P6912

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 1 mg substrate per tablet

Sigma-Aldrich

P6662

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 2 mg substrate per tablet

Sigma-Aldrich

P6787

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 3 mg substrate per tablet

Sigma-Aldrich

P8806

o-Phenylenediamine dihydrochloride

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

form

tablet

form

tablet

form

tablet

form

tablet

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in ELISA (enzyme-linked immunosorbent assay).[1][2][3]

Packaging

150 mg total tablet weight

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000479108
0000479108
0000438609
0000438609
0000391782

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