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P6998

Sigma-Aldrich

Protein Kinase A Catalytic Subunit β, Active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

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Synonym(s):
PKA Catalytic Subunit β, Active human, PKA-Cβ), cAMP-dependent protein kinase
Enzyme Commission number:
MDL number:

recombinant

expressed in baculovirus infected Sf9 cells

Quality Level

assay

≥80% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥10 nmol/min·mg

mol wt

protein apparent mol wt ~65 kDa (including N-terminal GST-tag of ~26kDa)

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKACB(5567)

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C8482P3738K3643
Sigma-Aldrich

P3738

Protein Kinase G Iβ human

Sigma-Aldrich

K3643

PKAcγ, active, GST tagged human

assay

≥80% (SDS-PAGE)

assay

>90% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥70% (SDS-PAGE)

Gene Information

human ... PRKACB(5567)

Gene Information

human ... PRKACA(5566)

Gene Information

human ... PRKG1(5592)

Gene Information

human ... PRKACG(5568)

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in E. coli

recombinant

expressed in baculovirus infected Sf9 cells

recombinant

expressed in baculovirus infected Sf9 cells

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

UniProt accession no.

P22694

UniProt accession no.

P17612

UniProt accession no.

Q13976

UniProt accession no.

P22612

General description

Protein kinase cAMP-activated catalytic subunit β (PRKACB) is one of the catalytic subunits of the serine/threonine kinase, protein kinase A. The gene encoding it is localized on human chromosome 1p36.1.

The molecular weight of about 65kDa is confirmed by the SDS-PAGE gel. The product was generated by expressing recombinant full-length human PKAcβ2 with an additional N-terminal GST tag by baculovirus in Sf9 insect cells. The gene accession number is NM_002731. The resulting protein is 351aa cAMP-dependent protein kinase catalytic subunit beta isoform 2 (NP_002722), or according to UniProt, Isoform 1 (P22694-1).

Application

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.

Biochem/physiol Actions

cAMP-dependent protein kinase (PKA; EC 2.7.1.37) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription.

Unit Definition

One unit will incorporate one nanomole of phosphate into the Crebtide (KRREILSRRPSYR) substrate per minute at 30 deg C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

Physical form

Supplied as a solution of 5 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Association between PKA gene polymorphism and NTDs in high risk Chinese population in Shanxi.
Wu J, et.al
International Journal of Clinical and Experimental Pathology, 6(12), 2968-2974 (2013)
Jian Wu et al.
International journal of clinical and experimental pathology, 6(12), 2968-2974 (2013-12-03)
This study aimed to investigate the single nucleotide polymorphisms (SNPs) of PKA and neural tube defects (NTDs) in Chinese population. A total of 183 NTDs cases and 200 healthy controls were used in this study. 7 selected single nucleotide polymorphism

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