P6998

Sigma-Aldrich

Protein Kinase A Catalytic Subunit β, Active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):
cAMP-dependent protein kinase, PKA Catalytic Subunit β, Active human, PKA-Cβ)
Enzyme Commission number:
MDL number:

Quality Level

recombinant

expressed in baculovirus infected Sf9 cells

assay

≥80% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

≥10 nmol/min·mg

mol wt

protein apparent mol wt ~65 kDa

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKACB(5567)

General description

Protein kinase cAMP-activated catalytic subunit β (PRKACB) is one of the catalytic subunits of the serine/threonine kinase, protein kinase A. The gene encoding it is localized on human chromosome 1p36.1.

Application

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.

Biochem/physiol Actions

cAMP-dependent protein kinase (PKA; EC 2.7.1.37) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription.

Unit Definition

One unit will incorporate one nanomole of phosphate into the Crebtide (KRREILSRRPSYR) substrate per minute at 30 deg C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

Physical form

Supplied as a solution of 5 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Association between PKA gene polymorphism and NTDs in high risk Chinese population in Shanxi.
Wu J, et.al
International Journal of Clinical and Experimental Pathology, 6(12), 2968-2974 (2013)
Jian Wu et al.
International journal of clinical and experimental pathology, 6(12), 2968-2974 (2013-12-03)
This study aimed to investigate the single nucleotide polymorphisms (SNPs) of PKA and neural tube defects (NTDs) in Chinese population. A total of 183 NTDs cases and 200 healthy controls were used in this study. 7 selected single nucleotide polymorphism...
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