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P7059

Sigma-Aldrich

Phosphate Buffered Saline

10× PBS for Western blots and IP

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Synonym(s):
Phosphate buffered saline, PBS
MDL number:
eCl@ss:
32129211
NACRES:
NA.25

grade

for molecular biology

Quality Level

sterility

0.2 μm filtered

form

liquid

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

pH

7.2-7.6(1x solution)

storage temp.

room temp

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This Item
806552806544P38135
SAFC

SAFC

806552

Phosphate Buffered Saline

SAFC

SAFC

806544

Phosphate Buffered Saline

sterility

0.2 μm filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

form

liquid

form

liquid

form

liquid

form

powder

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

pH

7.2-7.6(1x solution)

pH

7.4

pH

7.2

pH

7.4

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

General description

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Application

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

Components

10x PBS contains:
  • 30mM sodium phosphate
  • 1.5 M sodium chloride
  • 10.5 mM potassium phosphate

Preparation Note

Dilute 10x PBS to a 1x working solution using molecular biology grade water.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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