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P7059

Phosphate Buffered Saline

10X, liquid, pH 7.2-7.6 (1x solution), for molecular biology

Synonym(s):

Phosphate buffered saline, PBS

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1 L

Available to ship TODAYfromMILWAUKEE

$163.00

About This Item

UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25
MDL number:
MFCD00131855

$163.00

Available to ship TODAYDetails

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Product Name

Phosphate Buffered Saline, 10× PBS for Western blots and IP

grade

Molecular Biology

Quality Level

200

sterility

0.2 μm filtered

form

liquid

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

pH

7.2-7.6(1x solution)

storage temp.

room temp

General description

Phosphate Buffered Saline (PBS) is routinely used as a wash buffer in Western blot and immunoprecipitation (IP) procedures, as well as during sample preparation of cell lysates for use in general molecular biology applications.

Application

Suitable for use in wash buffers for Western blots and IP, as well as cell lysate sample preparation.

Preparation Note

10x PBS contains:
  • 30mM sodium phosphate
  • 1.5 M sodium chloride
  • 10.5 mM potassium phosphate
Dilute 10x PBS to a 1x working solution using molecular biology grade water.

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This Item
806552806544P4739
Phosphate Buffered Saline 10× PBS for Western blots and IP

Sigma-Aldrich

P7059

Phosphate Buffered Saline

Phosphate Buffered Saline pH 7.4, liquid, sterile-filtered, suitable for cell culture

SAFC

806552

Phosphate Buffered Saline

Phosphate Buffered Saline pH 7.2, liquid, sterile-filtered, suitable for cell culture

SAFC

806544

Phosphate Buffered Saline

Phosphate buffered saline with 5% nonfat milk, powder (dry milled), pH 7.3

Sigma-Aldrich

P4739

Phosphate buffered saline

technique(s)

immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

grade

Molecular Biology

grade

-

grade

-

grade

-

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

100

form

liquid

form

liquid

form

liquid

form

powder (dry milled)

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

-

pH

7.2-7.6(1x solution)

pH

7.4

pH

7.2

pH

7.3

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000545813
0000545813
0000519412
0000519412
0000492632

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Articles

Water for pH Measurement and Buffer Preparation

Learn how water impurities can affect pH. Water quality defined as pure water or Type 2 water is recommended to prepare buffer solutions or measure pH.

Jennifer L S Sporty et al.
Analytical chemistry, 82(15), 6593-6600 (2010-07-14)
A novel method for extracting butyrylcholinesterase (BuChE) from serum as a means of identifying and measuring nerve agent adducts to human BuChE is presented here. Antibutyrylcholinesterase monoclonal antibodies were conjugated to protein-G ferromagnetic particles and mixed with 500 microL serum
Sahar M Greish et al.
International journal of physiology, pathophysiology and pharmacology, 12(5), 134-146 (2020-11-24)
Hypertension can be induced by inhibiting nitric oxide synthesis with L-NAME, which also has a role in oxidative stress. Curcumin has strong antioxidant property. Our aim was to examine the possible preventive role of curcumin on renal dysfunction secondary to
Johan E Karlsson et al.
Drug metabolism and disposition: the biological fate of chemicals, 38(4), 705-714 (2010-01-15)
Membrane-bound transporter proteins play an important role in the efflux of drugs from cells and can significantly influence the pharmacokinetics of drug molecules. This study describes the production of large amounts of high-activity transporter membrane vesicles from human embryonic kidney
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Global Trade Item Number

SKUGTIN
P7059-1L04061834394297

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