P7119

Sigma-Aldrich

PGO Enzyme Preparation

1 G capsules

NACRES:
NA.54

Quality Level

form

powder

solubility

H2O: soluble capsule/100 mL at 25 °C, clear, colorless (pH 6.95-7.05)

storage temp.

2-8°C

General description

Each capsule contains 500 units of glucose oxidase (Aspergilus niger), 100 purpurogallin units of peroxidase (horseradish), and buffer salts. The reaction produces oxidized o-Dianisidine, which is brown. The intensity of the brown color measured at 425-475 nm is proportional to the original glucose concentration.

Application

PGO Enzymes preparation has been used:
  • in diet sampling and chemical analyses
  • to determine plasma and follicular fluid glucose levels
  • to measure glucose content in fractions of retrograded debranched rice starch

Packaging

10 caps in glass bottle

Biochem/physiol Actions

PGO Enzymes are used for the quantitative, enzymatic determination of glucose in aqueous solutions such as serum. The reactions are normally monitored at 425-475 nm utilizing o-dianisidine as a colorimetric substrate. Glucose oxidase methods eliminate the effects of interfering substances. Contents of one capsule includes 100 units peroxidase and 500 units glucose oxidase and buffer salts.

Suitability

Tested and found suitable for use in the quantitation of glucose.

Preparation Note

The PGO enzymes solution is prepared by adding the contents of 1 capsule of PGO enzymes to 100 mL of water in an amber bottle. Bottle should be inverted several times with gentle shaking to dissolve. PGO Enzymes solution should be stored at 2-8 °C. The solution is stable for up to 1 month unless turbidity develops, and for at least 6 months at –20 °C.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Joel I Jokinen et al.
Plants (Basel, Switzerland), 8(6) (2019-06-05)
Infection by holoparasitic plants typically causes decreases in host mass, thought to be primarily as a result of resource abstraction. Inverse relationships have been noted between the number of Orobanche spp. parasites infecting a host and their mass, suggesting that...
G B Penner et al.
Journal of dairy science, 92(4), 1725-1733 (2009-03-25)
This study was conducted to determine the effects of feeding Fermenten (Church and Dwight Co., Princeton, NJ) with or without dietary sucrose on ruminal fermentation, apparent total-tract nutrient digestibility, and nutrient utilization. Eight ruminally cannulated Holstein cows (163 +/- 55...
L O Chow et al.
Journal of dairy science, 91(4), 1534-1543 (2008-03-20)
Objectives of the study were to evaluate the effect of planting date on in vitro neutral detergent fiber digestibility (IVFD) of whole-crop barley (Hordeum vulgare) and its effects on productivity of lactating dairy cows. Two cultivars of barley were planted...
R Salehi et al.
Journal of dairy science, 99(5), 3584-3597 (2016-03-14)
The objectives were to determine the effects of supplemental fat (no oilseed vs. oilseed) during late gestation and the source of fat (canola vs. sunflower seed), on dry matter intake (DMI), plasma metabolite concentrations, milk production and composition, calf birth...
G B Penner et al.
Journal of dairy science, 92(6), 2767-2781 (2009-05-19)
The objective of the study was to investigate the fractional rate of volatile fatty acid (VFA) absorption and the expression of genes encoding for transporters and enzymes involved in the absorption and metabolism of VFA in ruminal tissue when cattle...

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