P7123

Sigma-Aldrich

Protein Kinase Cν (PKCν) Active human

recombinant, expressed in E. coli, ≥75% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):
Protein Kinase C nu, PKD3
MDL number:
NACRES:
NA.32

Quality Level

recombinant

expressed in E. coli

assay

≥75% (SDS-PAGE)

form

buffered aqueous glycerol solution

mol wt

protein apparent mol wt ~142 kDa

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PRKD3(23683)

Application

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide (KRREILSRRPSYR) per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.

Biochem/physiol Actions

PKCν, also known as PKD3, is a member of the protein kinase C (PKC) family of serine/threonine kinases that play critical roles in the regulation of cellular differentiation and proliferation in many cell types. PKCν is an important physiologic target of the B-cell receptor (BCR) and exhibits activation upon BCR engagement.

Physical form

Solution of 5 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol

WGK Germany

1

Certificate of Analysis
Certificate of Origin

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