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P7181

Polyethylene glycol solution

Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

Synonym(s):

PEG Solution, Polyethylene Glycol

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P7181-5X5ML

$119.00

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About This Item

MDL number:
MFCD00081839
UNSPSC Code:
12161902
PubChem Substance ID:
24898829
NACRES:
NA.75

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grade

Hybri-Max™

Quality Level

200

sterility

0.2 μm filtered

product line

BioReagent

form

solution

mol wt

average mol wt 1,450

concentration

50 % (w/v)

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

SMILES string

C(CO)O

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

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Polyethylene glycol solution Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

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P7181

Polyethylene glycol solution

Polyethylene glycol/ dimethyl sulfoxide solution Hybri-Max™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma

Sigma-Aldrich

P7306

Polyethylene glycol/ dimethyl sulfoxide solution

Polyethylene glycol solution 40 % (w/w) in H2O, average mol wt 8,000

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P1458

Polyethylene glycol solution

Polyethylene glycol solution BioUltra, Molecular Biology, 1,000, ~50% in H2O

Sigma-Aldrich

76293

Polyethylene glycol solution

form

solution

form

solution

form

-

form

liquid

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

mol wt

average mol wt 1,450

mol wt

average mol wt 1,450

mol wt

average mol wt 8,000

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, none detected, phosphatases, none detected, proteases, none detected

grade

Hybri-Max™

grade

Hybri-Max™

grade

-

grade

for molecular biology

General description

Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.[1]

Application

Polyethylene glycol solution has been used in the hybridization of the following:
  • irradiated camel fibroblast cells with A23 hamster cells[2]
  • mouse splenocytes with Sp2/0-Ag 14 mouse myeloma cells[3]
  • rabbit splenocytes and cell line 240E-1[4]

Recommended for use in a normal fusion protocol requiring 50% PEG.

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.

Biochem/physiol Actions

Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics.[1] PEG is commonly used as a lubricant. It′s application is also observed in chromatographic stationary phases.[5] PEG prevents crystal formation in emulsions.[6]

Packaging

Packaged in sealed ampules under nitrogen.

Preparation Note

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

212.0 °F - closed cup

flash_point_c

100 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
RNBK9585
RNBK0589
RNBH8345
RNBG7124
RNBG0388

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Development of Monoclonal Antibodies against CMP-N-Acetylneuraminate-beta-galactosamide-alpha-2, 3-sialyltransferase 1 (ST3Gal-I) Recombinant Protein Expressed in E. coli
Gupta AK, et al.
Biochemistry Research International, 2015(1), 1982-1982 (2015)
Characterization of a large panel of rabbit monoclonal antibodies against HIV-1 gp120 and isolation of novel neutralizing antibodies against the V3 loop
Qin Y, et al.
PLoS ONE, 10(6), e0128823-e0128823 (2015)
Retinol encapsulated into amorphous Ca2+ polyphosphate nanospheres acts synergistically in MC3T3-E1 cells
Muller WEG, et al.
European Journal of Pharmaceutics and Biopharmaceutics, 93(2), 214-223 (2015)
Construction of two whole genome radiation hybrid panels for dromedary (Camelus dromedarius): 5000 RAD and 15000 RAD
Perelman PL, et al.
Scientific reports, 8(1), 1982-1982 (2018)
Acoustic, thermal and molecular interactions of polyethylene glycol (2000, 3000, 6000)
Venkatramanan K, et al.
Physics Procedia, 70, 1052-1056 (2015)
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