MilliporeSigma
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P7280

Sigma-Aldrich

Poly-D-lysine hydrobromide

average mol wt 30,000-70,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture

Synonym(s):
PDL
Linear Formula:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.75

Quality Level

sterility

γ-irradiated

product line

BioReagent
BioXtra

form

lyophilized powder

mol wt

average mol wt 30,000-70,000

packaging

pkg of 5 X 5 mg
pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 50 mg/mL, clear, colorless

shipped in

ambient

storage temp.

−20°C

SMILES string

O=C(C)[C@@](NC)([H])CCCCN.[Br]

InChI

1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H

InChI key

MEXAGTSTSPYCEP-UHFFFAOYSA-N

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This Item
P6282P6407P9155
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

average mol wt 30,000-70,000

mol wt

70,000-150,000

mol wt

70,000-150,000

mol wt

30,000-70,000

packaging

pkg of 5 X 5 mg, pkg of 5 mg

packaging

pkg of 5 mg

packaging

pkg of 10 X 5 mg, pkg of 5 mg

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 50 mg/mL, clear, colorless

solubility

H2O: soluble 50 mL/vial, clear, colorless

solubility

H2O: soluble 50 mg/mL, clear, colorless

solubility

H2O: soluble 50 mg/mL, clear, colorless

Application

Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.

This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.

Biochem/physiol Actions

This product is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.

Components

Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.

Caution

Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.

Preparation Note

This product has a molecular weight of 30,000-70,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

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