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P7306

Polyethylene glycol/ dimethyl sulfoxide solution

Hybri-Max™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma

Synonym(s):

PEG/DMSO Solution, Polyethylene Glycol Solution, Solution of PEG and DMSO

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5 X 5 ML

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About This Item

MDL number:
MFCD00081839
UNSPSC Code:
12161902
PubChem Substance ID:
24898839
NACRES:
NA.75

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Product Name

Polyethylene glycol/ dimethyl sulfoxide solution, Hybri-Max™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma

InChI key

LYCAIKOWRPUZTN-UHFFFAOYSA-N

InChI

1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2

SMILES string

C(CO)O

grade

Hybri-Max™

sterility

0.2 μm filtered

product line

BioReagent

form

solution

mol wt

average mol wt 1,450

concentration

50 % (w/v)

technique(s)

cell culture | hybridoma: suitable

impurities

endotoxin, tested

storage temp.

2-8°C

Quality Level

200

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This Item
P7181P145876293
Polyethylene glycol/ dimethyl sulfoxide solution Hybri-Max™, average mol wt 1,450, 50 % (w/v), 0.2 μm filtered, BioReagent, suitable for hybridoma

Sigma-Aldrich

P7306

Polyethylene glycol/ dimethyl sulfoxide solution

Polyethylene glycol solution Hybri-Max™, 50 % (w/v), average mol wt 1,450, 0.2 μm filtered, BioReagent, suitable for hybridoma

Sigma-Aldrich

P7181

Polyethylene glycol solution

Polyethylene glycol solution 40 % (w/w) in H2O, average mol wt 8,000

Sigma-Aldrich

P1458

Polyethylene glycol solution

Polyethylene glycol solution BioUltra, Molecular Biology, 1,000, ~50% in H2O

Sigma-Aldrich

76293

Polyethylene glycol solution

form

solution

form

solution

form

-

form

liquid

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

mol wt

average mol wt 1,450

mol wt

average mol wt 1,450

mol wt

average mol wt 8,000

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, none detected, phosphatases, none detected, proteases, none detected

grade

Hybri-Max™

grade

Hybri-Max™

grade

-

grade

for molecular biology

Application

PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.


Recommended for use in a normal fusion protocol requiring 50% PEG and 10% DMSO.

Other Notes

Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) and 10% DMSO (v/v) in DPBS without Calcium.

Packaging

Packaged in sealed ampules under nitrogen.

Preparation Note

Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
RNBK7777
069K2320
097K2411
116K2327
062K2300

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Wen-Hung Chen et al.
Virology journal, 14(1), 189-189 (2017-10-04)
Dengue virus (DV) infection causes a spectrum of clinical diseases ranging from dengue fever to a life-threatening dengue hemorrhagic fever. Four distinct serotypes (DV1-4), which have similar genome sequences and envelope protein (E protein) antigenic properties, were divided. Among these
Indre Kucinskaite-Kodze et al.
Biomolecules, 10(7) (2020-07-12)
The pathogenicity of many bacteria, including Streptococcus pneumoniae, depends on pore-forming toxins (PFTs) that cause host cell lysis by forming large pores in cholesterol-containing cell membranes. Therefore, PFTs-neutralising antibodies may provide useful tools for reducing S. pneumoniae pathogenic effects. This
Shudan Wang et al.
Theranostics, 10(15), 6854-6874 (2020-06-20)
Repeated failures of "Aβ-lowering" therapies call for new targets and therapeutic approaches for Alzheimer's disease (AD). We propose to treat AD by halting neuronal death and repairing synapses using a BDNF-based therapy. To overcome the poor druggability of BDNF, we
Hong Chang et al.
Oncology letters, 22(5), 749-749 (2021-09-21)
The receptor tyrosine kinase, anexelekto (Axl) is involved in tumor cell growth, migration and invasion, and has been associated with chemotherapy resistance, which makes it an attractive target for cancer therapy. In total, six Axl-targeted monoclonal antibodies (mAbs) and two
Liliana R Loureiro et al.
Scientific reports, 8(1), 12196-12196 (2018-08-17)
Incomplete O-glycosylation is a feature associated with malignancy resulting in the expression of truncated glycans such as the sialyl-Tn (STn) antigen. Despite all the progress in the development of potential anti-cancer antibodies, their application is frequently hindered by low specificities
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