One potential replacement is item number P7405. This is the HBr form, making it more water-soluble. However, it may be necessary to conduct tests to evaluate its compatibility with your specific cell line. If needed, there are methods to prepare the solution and subsequently remove the HBr through dialysis. This polymer is of the same size (>300,000), gamma-irradiated, and has been tested for cell culture.
P7405
Poly-ᴅ-Lysine Hydrobromide
synthetic, mol wt >300,000, powder, γ-irradiated, suitable for cell culture, BioXtra
Synonym(s):
PDL
Sign In to View Organizational & Contract Pricing.
About This Item
Linear Formula:
D-Lys-(D-Lys)n-D-Lys · xHBr
CAS Number:
UNSPSC Code:
12352202
PubChem Substance ID:
NACRES:
NA.75
MDL number:
Biological source:
synthetic (chemical)
Form:
lyophilized powder
Technique(s):
cell culture | mammalian: suitable
Concentration:
0.016—0.032 mmol lysine
Mol wt:
>300,000
Skip To
Product Name
Poly-D-lysine hydrobromide, mol wt >300,000, lyophilized powder, γ-irradiated, BioReagent, suitable for cell culture
SMILES string
O=C(C)[C@@](NC)([H])CCCCN.[Br]
InChI
1S/C6H14N2O2.BrH/c7-4-2-1-3-5(8)6(9)10;/h5H,1-4,7-8H2,(H,9,10);1H
InChI key
MEXAGTSTSPYCEP-UHFFFAOYSA-N
biological source
synthetic (chemical)
sterility
γ-irradiated
product line
BioReagent, BioXtra
form
lyophilized powder
mol wt
>300,000
packaging
pkg of 5 mg
concentration
0.016—0.032 mmol lysine
technique(s)
cell culture | mammalian: suitable
surface coverage
4 μg/cm2
solubility
H2O: soluble 50 mg/mL, clear, colorless
shipped in
ambient
storage temp.
−20°C
Quality Level
Looking for similar products? Visit Product Comparison Guide
1 of 4
This Item | P7280 | P6407 | P0899 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility γ-irradiated | sterility γ-irradiated | sterility γ-irradiated | sterility - |
| biological source synthetic (chemical) | biological source synthetic (organic) | biological source synthetic (organic) | biological source - |
| concentration 0.016—0.032 mmol lysine | concentration 0.016—0.032 mmol lysine | concentration 0.016—0.032 mmol lysine | concentration - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| mol wt >300,000 | mol wt average mol wt 30,000-70,000 | mol wt 70,000-150,000 | mol wt 70,000-150,000 |
Analysis Note
This product has a molecular weight >300,000 and is cell culture tested and gamma-irradiated. To remove the HBr, dissolve this product in a neutral buffer and dialyze to remove the salts. In general, to use this product as an attachment factor, add 50 mL of sterile tissue culture grade water to 5 mg of poly-lysine, and aseptically coat the surface with 1 mL per 25 cm2 of solution. After 5 minutes, remove the solution through aspiration and thoroughly rinse the surface. Let dry for two hours before introducing cells and medium.
Application
Poly-D-lysine hydrobromide has been used :
- in preparing the surface of coverslips for cell attachment[1] [2]
- in coating glass-bottom dishes for live cell imaging of primary cortical neuronal cells[3]
- in coating 24-well plates for the isolation of primary astrocytes to study the impact of melanoma-derived exosomes on the reprogramming of stromal cells within the metastatic microenvironment[4]
- for coating 16-well chamber slides to study the effects of maternal immune activation on behavioral impairments and alterations in cytokine and synaptic protein expression in the cerebellum[5]
Poly-D-lysine polymers can be used in preparing surfaces for cell attachment. The D-lysine polymers can also be used with cells that digest poly-L-lysine polymers and cause an excessive uptake of L-lysine.
This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
This product is recommended as a cell culture substratum when using 0.5 - 1.0 mL of a 0.1 mg/mL solution to coat 25 cm2. Lower molecular weight versions of the product are less viscous, but high more molecular weight versions provide more attachment sites per molecule.
Biochem/physiol Actions
Poly-D-Lysine Hydrobromide (PDL) is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.[6] PDL increases the number of positively charged cell binding sites post-absorption to the culture surface.[7] PDL coating promotes the attachment of cells to solid surfaces that usually grow in suspension.[8]
Poly-D-lysine (PDL) hydrobromide is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. After absorption to the culture surface, poly-D-lysine increases the number of positively charged cell binding sites.
Disclaimer
Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.
General description
Poly-D-Lysine Hydrobromide (PDL) is a cationic polymer composed of lysine residues with an associated hydrobromide molecule per lysine unit. The presence of the hydrobromide molecule introduces a distinctive property to the poly-D-lysine structure allowing the poly-D-lysine to adopt a crystalline form, rendering it soluble in aqueous solutions.
Other Notes
Poly-D-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-D-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the ß structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Melanoma-derived extracellular vesicles instigate proinflammatory signaling in the metastatic microenvironment
Lahav TG, et al.
International Journal of Cancer. Journal International Du Cancer, 145(9), 2521-2534 (2019)
Deficiency of ROS-Activated TRPM2 Channel Protects Neurons from Cerebral Ischemia-Reperfusion Injury through Upregulating Autophagy
Hu X, et al.
Oxidative Medicine and Cellular Longevity (2021)
CRISPR-Mediated Activation of ?V Integrin Subtypes Promotes Neuronal Differentiation of Neuroblastoma Neuro2a Cells
Riccardi S, et al.
Frontiers in genome editing, 4 (2022)
Maternal Immune Activation Causes Behavioral Impairments and Altered Cerebellar Cytokine and Synaptic Protein Expression
Pendyala G, et al.
Neuropsychopharmacology, 42, 1435?1446-1435?1446 (2017)
Barbamide Displays Affinity for Membrane-Bound Receptors and Impacts Store-Operated Calcium Entry in Mouse Sensory Neurons
Hough A, et al.
Marine drugs, 21(2), 110-110 (2023)
Articles
Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.
Protocols
Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.
Related Content
-
Have you considered any alternative products that have been tested and found suitable for the primary culture of cortical neurons? This inquiry arises as the expected delivery date for the previously ordered A-003-E Poly-D-lysine has been postponed to April 2022, and the current stock is expected to be depleted soon.
1 answer-
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service