No, this product does not contain a His-tag. See the link below for additional product information: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/394/024/p7582dat.pdf
P7582
Amino-terminal FLAG-BAP™ Fusion Protein
Synonym(s):
Amino-terminal Fusion Protein, BAP Fusion Protein, FLAG-BAP Fusion Protein
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P7582-100UG
$392.00
$392.00
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This Item | P7457 | P5975 | F3290 |
|---|---|---|---|
| mol wt ~49 kDa | mol wt ~49 kDa | mol wt ~49 kDa | mol wt 1012.97 Da |
| form liquid | form liquid | form - | form lyophilized powder |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in wet ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Amino-terminal FLAG-BAP™ Fusion Protein is a 467 amino acid N-terminal FLAG fusion protein of E. coli bacterial alkaline phosphatase (BAP) with a calculated molecular mass of 49.3kDa. The N-terminal FLAG-BAP Fusion Protein migrates as a 45−55kDa band by SDS-PAGE depending on electrophoresis conditions. FLAG-BAP™ Fusion Protein has biotechnological applications and is used in the screening of recombinant fusion proteins.[1]
The Amino-terminal FLAG-BAP Fusion Protein is a control prorein used to confirm the funtional integrity of the anti-FLAG M1 and M2 monoclonal antibodies in applications such as western blotting, immunoprecipitation, ELISA, electron microscopy, and FACS.
Application
Amino-terminal FLAG-BAP™ Fusion Protein has been used as a standard in enzyme-linked immunosorbent assay (ELISA) for osteopontin quantification[2] and in western blotting based quantification of Flag-Wnt8[3] and Drosophila melanogaster transcription factor interactome.[4]
Learn more product details in our FLAG® application portal.
Physical form
Protein is supplied in 10 mM Tris, 120 mM NaCl,
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.
0.05 mM ZnCl2 in 50% glycerol, pH 8.0.
Other Notes
Control protein
Legal Information
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG-BAP is a trademark of Sigma-Aldrich Co. LLC
Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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BAP-fusion: A versatile molecular probe for biotechnology research
Biotechnology: Research, Technology and Applications, 327-345 (2008)
A Comprehensive Drosophila melanogaster Transcription Factor Interactome
Shokri L, et al.
Cell Reports, 27(3), 955-970 (2019)
Exploring the separation power of mixed-modal resins for purification of recombinant osteopontin from clarified Escherichia coli lysates
Guo S, et al.
Biotechnology Progress, 35(1), e2722-e2722 (2019)
Noggin4 is a long-range inhibitor of Wnt8 signalling that regulates head development in Xenopus laevis
Eroshkin FM, et al.
Scientific Reports, 6, 23049-23049 (2016)
Daniela Castiglia et al.
Biotechnology for biofuels, 9, 154-154 (2016-07-28)
Biofuels production from plant biomasses is a complex multi-step process with important economic burdens. Several biotechnological approaches have been pursued to reduce biofuels production costs. The aim of the present study was to explore the production in tobacco plastome of
Articles
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
Protocols
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
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Técnicas, reactivos y protocolos de purificación de proteínas para purificar proteínas recombinantes utilizando métodos como la cromatografía de intercambio iónico, de exclusión por tamaño y de afinidad.
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