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MilliporeSigma

P7643

Sigma-Aldrich

PIPES dipotassium salt

≥99% (titration)

Synonym(s):

1,4-Piperazinediethanesulfonic acid dipotassium salt, Piperazine-1,4-bis(2-ethanesulfonic acid) dipotassium salt

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About This Item

Empirical Formula (Hill Notation):
C8H16K2N2O6S2
CAS Number:
Molecular Weight:
378.55
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NA.25

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Quality Level

assay

≥99% (titration)

form

crystalline powder

useful pH range

6.1-7.5

pKa (25 °C)

6.8

solubility

water: 0.25 g/mL, clear, colorless

application(s)

diagnostic assay manufacturing

SMILES string

[K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;;/h1-8H2,(H,11,12,13)(H,14,15,16);;/q;2*+1/p-2

InChI key

BJQYFCAMUXGZFN-UHFFFAOYSA-L

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PIPES ≥99% (titration)

P6757

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PIPES BioXtra, ≥99% (titration)

P8203

PIPES

assay

≥99% (titration)

assay

≥99% (titration)

assay

≥99% (titration)

assay

≥99% (titration)

Quality Level

200

Quality Level

400

Quality Level

200

Quality Level

400

form

crystalline powder

form

crystalline powder

form

crystalline powder

form

powder

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

solubility

water: 0.25 g/mL, clear, colorless

solubility

1 M NaOH: 20 + 80 mL g, clear, colorless

solubility

water: 0.5 g/mL, clear, colorless

solubility

1 M NaOH: 0.5 M at 20 °C, clear, colorless

useful pH range

6.1-7.5

useful pH range

6.1-7.5

useful pH range

6.1-7.5

useful pH range

6.1-7.5

General description

PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.[1]

Application

PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding.[2] It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells.[3] It is also suitable for use as buffer for purification of tubulin.[4]

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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