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P7892

Sigma-Aldrich

Phenyl-Sepharose CL-4B

extent of labeling: ~40 μmol per mL

Synonym(s):
Phenyl-Agarose
MDL number:
NACRES:
NA.56

form

suspension

Quality Level

extent of labeling

~40 μmol per mL

matrix

agarose, 4% cross-linked

matrix activation

epichlorohydrin

matrix attachment

hydroxyl

matrix spacer

3 atoms

particle size

45-165 μm

capacity

15-20 mg/mL binding capacity (human serum albumin)
3-5 mg/mL binding capacity (β-lactoglobulin)

storage temp.

2-8°C

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General description

P7892-200ML′s updated product number is GE17-0810-01

Application

Phenyl Sepharose® is used in protein chromatography, affinity chromatography, hydrophobic interaction media, resins and separation media. Phenyl Sepharose® has been used in studies that contributed to improving industrial applications in additives in detergents and feed industries. Phenyl Sepharose® has also been used to study microbial communities inhabiting hypersaline environments.

Packaging

10, 50, 200 mL in glass bottle

Physical form

aqueous ethanol suspension
Suspension in 20% ethanol

Legal Information

Sepharose is a registered trademark of Cytiva

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 3

Flash Point(F)

96.8 °F

Flash Point(C)

36 °C

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

O Ploux et al.
The Biochemical journal, 283 ( Pt 2), 327-331 (1992-04-15)
The 8-amino-7-oxopelargonate synthase [6-carboxyhexanoyl-CoA:L-alanine carboxyhexanoyltransferase (decarboxylating); EC 2.3.1.47] from Bacillus sphaericus involved in biotin biosynthesis was purified from an Escherichia coli overproducing strain. The purification afforded an electrophoretically homogeneous enzyme with a specific activity of 0.67 unit/mg. The purified enzyme
S K Shenoy et al.
The Journal of biological chemistry, 272(28), 17867-17872 (1997-07-11)
The cDNA encoding the smallest membrane-anchoring subunit (QPs3) of bovine heart mitochondrial succinate-ubiquinone reductase was cloned and sequenced. This cDNA is 1330 base pairs long with an open reading frame of 474 base pairs that encodes the 103 amino acid
W Kroutil et al.
Journal of biotechnology, 61(2), 143-150 (1998-07-09)
A highly enantioselective, soluble epoxide from Nocardia sp. EH1 was purified to homogeneity via a four-step procedure: (i) hydrophobic interaction chromatography on Phenyl Sepharose CL-4B, (ii) anion exchange chromatography on SOURCE 30Q, followed by (iii) a second hydrophobic interaction chromatography
Sheref S Mansy et al.
Nature, 454(7200), 122-125 (2008-06-06)
Contemporary phospholipid-based cell membranes are formidable barriers to the uptake of polar and charged molecules ranging from metal ions to complex nutrients. Modern cells therefore require sophisticated protein channels and pumps to mediate the exchange of molecules with their environment.
Purification and properties of hepatic glutamine synthetases from the ureotelic gulf toadfish, <I>Opsanus beta</I>.
Walsh, P.J., et al.
Comp. Biochem. Physiol., B: Comp. Biochem., 115(4), 523-532 (1996)

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