P8038

Proteinase, bacterial

Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

• Synonym(s): Proteinase from Bacillus licheniformis, Alkaline Protease, Protease from Bacillus licheniformis, Subtilo peptidase A
• CAS Number: 9014-01-1
• Enzyme Commission number: 3.4.21.62 (BRENDA, IUBMB)
• EC Number: 232-752-2
• MDL number: MFCD00132092
• eCl@ss: 32160410
• NACRES: NA.54

Properties

• type: Type XXIV
• Quality Level: 300
• form: lyophilized powder
• specific activity: ≤0.05 Kunitz units/mg solid RNase; ≤5.0 Kunitz units/mg solid DNase; 7.0-14.0 units/mg solid
• mol wt: 27 kDa
• purified by: crystallization
• shipped in: wet ice
• storage temp.: −20°C

Description

Specificity

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.

Application

The enzyme from Sigma has been used to optimize release of all mitochondrial populations from homogenized ventricular tissue of rat heart. It has also been used in the pre-hybridisation treatment of formalin fixed, paraffin wax-embedded liver specimens for detecting human and viral DNA.

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. Product P8038, also known as Subtilisin Carlsberg, has been used to hydrolyze cardiac cells to study the silencing of cardiac mitochondrial NHE1.

Packaging

50, 100, 250 mg in glass bottle

1 g in glass bottle

Biochem/physiol Actions

Proteinase catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Physical properties

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Analysis Note

Amino acid analysis and isoelectric focusing electrophoresis consistent with subtilisin Carlsberg.

Safety Information

• Pictograms: GHS05,GHS07,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H302 - H315 - H318 - H334 - H335 - H410
• Precautionary Statements: P273 - P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Target Organs: Respiratory system
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves