P8045

Protein G-Biotin from Streptococcus sp.

recombinant, expressed in unspecified host, lyophilized powder

• MDL number: MFCD00132111
• NACRES: NA.46

Properties

• recombinant: expressed in unspecified host
• Quality Level: 200
• conjugate: biotin conjugate
• form: lyophilized powder
• composition: Protein, ~90% biuret
• extent of labeling: 2-4 mol d-biotin per mol protein
• technique(s): direct ELISA: 1:70,000 using human IgG
• storage temp.: 2-8°C

Description

General description

Protein G binds to antibodies reversibly and thus, it is considered in antibody purification. It is a 20 kDa protein.

Protein G is an immunoglobulin (IgG) specific bacterial cell wall protein isolated from Streptococci (group G). Native proteins G consist of two IgG-binding domains and albumin and cell surface binding sites. Biotin is a water soluble vitamin that acts as a coenzyme for carboxylase enzyme reactions. Biotin deficiency results in deregulation of metabolic pathways and various physiological impairments. Biotin deficiency is known to cause birth anomalies indicating its important role in development and differentiation of embryos.

Specificity

Binds IgG only from most mammalian species except cat; binds chicken IgG.

Application

Protein G-Biotin from Streptococcus sp. has been used during incubation for synergistic immunocapture of antigen which was performed using streptavidin-coated magnetic beads.

Protein G-Biotin from Streptococcus sp. has been used in electrochemical biosensing, for the attachment of antibodies with different specificities.

Packaging

250 μg

1 mg

Package size based on protein content

Other Notes

Truncated - lacks albumin, Fab and membrane binding sites.

Preparation Note

Prepared using biotinamidocaproate N-hydroxysuccinimide which incorporates an aminocaproyl spacer.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.