P8147

Sigma-Aldrich

Platelet-Derived Growth Factor from human platelets

PDGF, 1 μg/vial, lyophilized powder, suitable for cell culture

Synonym(s):
PDGF
MDL number:
NACRES:
NA.77
Pricing and availability is not currently available.

Quality Level

biological source

human

assay

≥95% (SDS-PAGE)

form

lyophilized powder

potency

0.5-10.0 ng/mL EC50

quality

endotoxin tested

mol wt

glycoprotein 28-31 kDa

packaging

pkg of 1 μg
pkg of 1 vial

storage condition

avoid repeated freeze/thaw cycles

application(s)

cell culture | mammalian: suitable

impurities

≤1 EU/μg

UniProt accession no.

storage temp.

−20°C

Gene Information

human ... PDGFA(5154), PDGFB(5155)

General description

Platelet-derived growth factor (PDGF) is expressed as homo and heterodimers: PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD. The PDGF gene is highly conserved and is mapped to human chromosome 7p22.3. The active form of PDGF exists as disulfide-linked dimers. It consists of cystine-knot-fold growth factor domain, which is highly conserved, except for the N-terminal prodomain, which shows variation in length among the isoforms.

Application

PDGF is a chemoattractant for fibroblasts, smooth muscle cells, monocytes and neutrophils. Also, it acts on fibroblasts and other cells that initiate healing and tissue restoration.

Biochem/physiol Actions

Platelet-derived growth factor (PDGF), not only functions as a growth factor, but also participates in chemotaxis for triggering cell movement. Elevated levels of placental PDGF is observed during the late first and third-trimester pregnancies. PDGF is associated with wound healing. PDGFRα (PDGF receptor α ) phosphorylation, mediated by PDGFA activates pathways associated with cancer. PDGFA has been indicated to induce growth and metastasis in human hepatocellular carcinoma. Vascular endothelial growth factor expression is regulated by PDGF-AA autocrine signaling and is known to promote tumor angiogenesis in vivo. PDGF-BB isomer is found to be critical in angiogenesis and is highly expressed in tumor, mediating oncogenic pericyte recruitment. PDGF-BB is an important regulator of bone homeostasis, repair and regeneration.
The principal mitogen found in mammalian serum, released from platelets during clot formation. Elicits mitogenesis of mesoderm-derived cells, increased extracellular matrix synthesis, and chemotaxis and activation of neutrophils, monocytes and fibroblasts. Mitogenic for dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells and chondrocytes. Appears to interact with transforming growth factor-β1 in accelerating wound healing. However, platelet-derived growth factor (PDGF) may also be pathogenic in arteriosclerosis and neoplasia.

Other Notes

Isolated from human platelets, predominantly containing two covalently linked subunits (A and B).

Physical form

Lyophilized from a 0.2-μm filtered solution in 40% acetonitrile plus 0.1% TFA

Analysis Note

The mitogenic activity is tested in culture using Swiss 3T3 cells or NR6-3T3 fibroblasts.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Angiogenesis during human extraembryonic development involves the spatiotemporal control of PDGF ligand and receptor gene expression
Holmgren L, et al.
Development, 113(3), 749-754 (1991)
Clinical Findings Associated with a De Novo Partial Trisomy 10p11. 22p15. 3 and Monosomy 7p22. 3 Detected by Chromosomal Microarray Analysis
Kohannim O, et al.
Case Reports in Genetics, 2011 (2011)
Platelet-derived growth factor-AA is an essential and autocrine regulator of vascular endothelial growth factor expression in non-small cell lung carcinomas
Shikada Y, et al.
Cancer Research, 65(16), 7241-7248 (2005)
Hong Yue et al.
Arteriosclerosis, thrombosis, and vascular biology, 39(2), 263-275 (2018-12-21)
Objective- Dysregulated proliferation of vascular smooth muscle cells (VSMC) plays an essential role in neointimal hyperplasia. CD36 functions critically in atherogenesis and thrombosis. We hypothesize that CD36 regulates VSMC proliferation and contributes to the development of obstructive vascular diseases. Approach...
Overexpression of platelet-derived growth factor-BB increases tumor pericyte content via stromal-derived factor-alpha CXCR4 axis
Song N, et al.
Cancer Research, 69(15), 6057-6064 (2009)
Articles
Platelet-Derived Growth Factor is the principal mitogen found in mammalian serum and is released from platelets during clot formation.
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