Skip to Content
MilliporeSigma
All Photos(4)

Key Documents

P8169

Sigma-Aldrich

Phytagel

Phytagel™

BioReagent, suitable for plant cell culture, powder

Synonym(s):

Gellan Gum, Agar substitute gelling agent

Sign Into View Organizational & Contract Pricing

Select a Size

100 G
$68.60
250 G
$122.00
500 G
$246.00
1 KG
$413.00
5 KG
$1,720.00

$68.60


In StockDetails


Request a Bulk Order

Select a Size

Change View
100 G
$68.60
250 G
$122.00
500 G
$246.00
1 KG
$413.00
5 KG
$1,720.00

About This Item

CAS Number:
EC Number:
UNSPSC Code:
10171502
NACRES:
NA.72

$68.60


In StockDetails


Request a Bulk Order

product line

BioReagent

form

powder

technique(s)

cell culture | plant: suitable

application(s)

agriculture

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
A3301G1910A9799
Agargel™ suitable for plant cell culture, powder

A3301

Agargel

Agar High gel strength, suitable for plant cell culture

A9799

Agar

form

powder

form

powder

form

-

form

powder

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

application(s)

agriculture

technique(s)

cell culture | plant: suitable

technique(s)

cell culture | plant: suitable

technique(s)

-

technique(s)

cell culture | plant: suitable

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

product line

BioReagent

product line

-

product line

-

product line

-

General description

Typical working concentration: 1.5-2.5 g/L in plant tissue culture media; up to 10 g/L in microbiological media. Phytagel requires the presence of cations (especially divalent) for gelling to occur. Concentrations of calcium and magnesium contained in most plant tissue culture media are typically sufficient for gelation. Low-salt media formulations, especially those used in microbiological applications, may require supplementation with additional calcium or magnesium salts (e.g., CaCl2 or MgSO4) or higher concentrations of Phytagel.

Application

Phytagel has been used:
  • in the solidification of culture medium for root assays in Arabidopsis thaliana[1]
  • as a component of Solid Paul′s medium for Agrobacterium culture[2]
  • as a component of root induction medium in Ilex dumosa tissue culture[3]

Biochem/physiol Actions

Phytagel is intended for use in place of agar and other gelling agent for plant tissue culture. Phytagel has been shown to be a superior substitute for tissue-culture-grade agar in the micropropagation of banana plantlets.

Other Notes

An agar substitute produced from a bacterial fermentation composed of glucuronic acid, rhamnose and glucose. It produces a clear, colorless, high strength gel which aids in detection of microbial contamination.

Reconstitution

To prevent clumping, add slowly to rapidly stirring medium prior to heating.

Legal Information

Phytagel is a trademark of Sigma-Aldrich Co. LLC

related product

Storage Class

11 - Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

1–4 of 4 Questions  
  1. What is the sulfate content, if any, of Phytagel?

    1 answer
    1. Elemental analysis of this material has not been performed. The sulfate content is not determined.

      Helpful?

  2. Buongiorno. Vorrei sapere se il Phytagel è Gellano ad alto o a basso grado di acilazione

    1 answer
    1. This product is characterized as having a low acyl content.

      Helpful?

  3. What is the molecular weight of one repeating unit?

    1 answer
    1. The molecular weight is not determined. However, the constituent sugars of Phytagel are glucose, glucuronic acid and rhamnose in the molar ratio of 2:1:1.

      Helpful?

  4. What is the gelling strength of Phytagel P8169

    1 answer
    1. The Gel Strength for Phytagel (P8169) is 700 g/cm2 (tested at 0.5%).

      Helpful?

Reviews

Active Filters

  1. 1 Ratings-Only Review

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service