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P8287

o-Phenylenediamine dihydrochloride

chromogenic, tablet

Synonym(s):

OPD, 1,2-Phenylenediamine tablet, OPD Tablet

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50 TABS

$277.00

100 TABS

$473.00

$277.00

Available to ship onJanuary 07, 2026Details

About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
615-28-1
Molecular Weight:
181.06
NACRES:
NA.83
PubChem Substance ID:
24898741
UNSPSC Code:
12352204
MDL number:
MFCD00012966
Beilstein/REAXYS Number:
3912045

Key Documents

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Product Name

o-Phenylenediamine dihydrochloride, tablet, 10 mg substrate per tablet

Quality Level

300

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

form

tablet

mp

258 °C (dec.) (lit.)

storage temp.

2-8°C

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o-Phenylenediamine dihydrochloride tablet, 10 mg substrate per tablet

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Sigma-Aldrich

P8412

o-Phenylenediamine dihydrochloride

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

200

form

tablet

form

tablet

form

tablet

form

tablet

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

Application

o-Phenylenediamine dihydrochloride has been used as a substrate for horseradish-peroxidase-conjugated secondary antibody in enzyme-linked immunosorbent assay (ELISA).[1][2][3]

General description

o-Phenylenediamine (o-PD) is a colorless and nonfluorescent molecule.[4]
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Preparation Note

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

signalword

Warning

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
0000464699
0000519389
0000519389
0000464699
0000486085

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Antibodies (Basel, Switzerland), 9(1) (2020-03-04)
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Malaria journal, 18(1), 227-227 (2019-07-10)
Analysis of anti-malarial antibody responses has the potential to improve characterization of the variation in exposure to infection in low transmission settings, where conventional measures, such as entomological estimates and parasitaemia point prevalence become less sensitive and expensive to measure.
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