P8371

Anti-Protein Kinase Cβ2 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-PKCβ2
• MDL number: MFCD00162771
• NACRES: NA.44

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human, mouse, rat
• technique(s): western blot: 2 μg/mL using recombinant human PKC beta II
• UniProt accession no.: P05771
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: human ... PRKCB(5579) ; mouse ... Prkcb(18751) ; rat ... Prkcb(25023)

Description

General description

Protein Kinase C (PKC, 76-93 kD) is a family of Ser/Thr specific protein kinases that perform key functions in numerous signalling pathways, in biological systems, through their various isoforms. The conventional PKC isoforms (cPKC) are PKC-α, β1, β2 and γ; activated by phosphatidylserine, calcium or phorbol esters. Proteolysis of PKC ^{in vivo} is thought to be mediated by calpains I and II. Calpains cleave PKC in the V3 hinge region to produce two distinct fragments, one comprising the N-terminal regulatory domain (30 kD) and the other fragment containing the C-terminal kinase domain (50 kD) that is catalytically active. Multiple functions such as, cellular and vascular regulations, angiogenesis, cell growth, apoptosis, changes in basement membrane thickness, extracellular matrix organisation, MAPK signalling, are attributed to PKC isoforms. These varied functions implicate PKC isoforms in cardiac hypertrophies and diabetic nephropathy and cardiovascular complications.
Anti-Protein Kinase C β2 antibody specifically recognises PKC β2 (80 kDa).

Immunogen

synthetic peptide corresponding to amino acid residues 654-665 from human PKCβ2.

Application

Anti-Protein Kinase C β2 antibody may be used for detection by immunoblotting at a working concentration of 2μg/mL.

Physical form

Solution in phosphate buffered saline containing 1.0 mg/mL BSA and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable