Upon the addition of crystalline serum albumin (10 mg/mL) and storage at -15°C, solutions of this product in water are stable for several months. Additional information can be found in the Methods in Enzymology XIV, 202 (1969).
P8398
Phospholipase D from cabbage
Type IV, lyophilized powder, ≥100 units/mg solid
Synonym(s):
Lecithinase D, Phosphatidylcholine phosphatidohydrolase, Phospholipase D from white cabbage
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P8398-500UN
$241.00
P8398-2.5KU
$937.00
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type
Type IV
Quality Level
form
lyophilized powder
specific activity
≥100 units/mg solid
composition
Protein, ~25%
storage temp.
−20°C
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This Item | P4912 | P5542 | P7633 |
|---|---|---|---|
| specific activity ≥100 units/mg solid | specific activity ≥150 units/mg solid | specific activity ≥1,000 units/mg protein (Lowry) | specific activity 10-50 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 300 | Quality Level 200 |
| type Type IV | type Type VII | type - | type Type I |
| composition Protein, ~25% | composition - | composition - | composition protein, 20-40% |
General description
Application
Phospholipase D (PLD) is used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation[2]. Furthermore, PLD is used in purification and kinetic studies[3].
The enzyme has been used for the preparation of Bodipy-phosphatidylcholine during the preparation of fluorescently labelled lipids.[4]
Biochem/physiol Actions
Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Phospholipase D from cabbage is widely used in biocatalytic transphosphatidylation reactions.[5]
The amino acid sequence of cabbage PLD has 83% and 80% identity with Arabidopsis PLDα and castor bean PLD[6]. It hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Analysis Note
Protein determined using biuret.
Other Notes
One unit will liberate 1.0 μmol of choline from L-α-phosphatidylcholine (egg yolk) per hr at pH 5.6 at 30 °C.
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signalword
Danger
hcodes
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Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
ppe
Eyeshields, Gloves, type N95 (US)
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T T Allgyer et al.
Biochemistry, 18(24), 5348-5353 (1979-11-27)
Phospholipase D has been purified 680-fold from an acetone powder of savoy cabbage in an overall yield of 30%. The purification involves solubilization of the acetone powder in a Ca2+-containing buffer and subsequent ammonium sulfate fractionation. Gel filtration on Sephadex
Alexandra Lerchner et al.
Protein engineering, design & selection : PEDS, 19(10), 443-452 (2006-07-18)
In addition to hydrolysis of glycerophospholipids, phospholipases D (PLDs) catalyze the head group exchange. The molecular basis of this transphosphatidylation potential, which strongly varies for PLDs from different sources, is unknown hitherto. Recently, the genes of two PLD isoenzymes from
D U Kim et al.
Biochimica et biophysica acta, 1437(3), 409-414 (1999-04-02)
We cloned and expressed a full-length cDNA encoding a phospholipase D of type alpha (PLDalpha) from cabbage. Analysis of the cDNA predicted an 812-amino-acid protein of 92.0 kDa. The deduced amino acid sequence of cabbage PLD has 83% and 80%
Cindy Y Jao et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(36), 15332-15337 (2009-08-27)
Choline (Cho)-containing phospholipids are the most abundant phospholipids in cellular membranes and play fundamental structural as well as regulatory roles in cell metabolism and signaling. Although much is known about the biochemistry and metabolism of Cho phospholipids, their cell biology
Susanne Stumpe et al.
The FEBS journal, 274(10), 2630-2640 (2007-04-25)
Phospholipases D play an important role in the regulation of cellular processes in plants and mammals. Moreover, they are an essential tool in the synthesis of phospholipids and phospholipid analogs. Knowledge of phospholipase D structures, however, is widely restricted to
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