P8458

Sigma-Aldrich

Anti-Protein Kinase Cε antibody produced in rabbit

whole antiserum

Synonym(s):
Anti-PKC ε
MDL number:
NACRES:
NA.44

biological source

rabbit

Quality Level

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

mol wt

antigen 90 kDa

contains

15 mM sodium azide

species reactivity

rat

application(s)

dot blot: 1:50,000
microarray: suitable
western blot: 1:15,000 using rat brain extract

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

rat ... Prkce(29340)

General description

Protein Kinase C (PKC) is a family of serine/threonine (Ser/Thr)-specific protein kinases. Protein kinase Cε (PKCε) is a member of the novel subgroup. The PKCε isoenzyme appears to be widely expressed in rat brain, lung, skin, liver, spleen, thymus, embryonic rat fibroblasts and in low levels, in certain mouse fibroblasts.

Specificity

Anti-Protein Kinase C ε (PKC ε) reacts in immuno-blotting with PKC ε (90 kDa polypeptide) from rat brain extract

Immunogen

synthetic peptide, corresponding to the C-terminal variable (V5) region (amino acids 726-737) of PKC ε.

Application

Anti-Protein Kinase Cε antibody produced in rabbit may be used in:
  • western blot
  • immunoprecipitation
  • enzyme linked immunosorbent assay (ELISA)
  • immunohistochemistry
  • chemiluminescence detection systems

Anti-Protein Kinase C epsilon antibody may be used:
  • for detection of PKCepsilon in whole cell or membrane extracts of HUVEC cells when stimulated with PMA
  • in indirectdot blot
  • for protein microarray

Biochem/physiol Actions

Protein Kinase C perform key functions in numerous signalling pathways, in biological systems, through their various isoforms. The PKC isoforms are activated by phosphatidylserine, calcium or phorbol esters. Proteolysis of PKC in vivo is thought to be mediated by calpains I and II. Multiple functions such as, cellular and vascular regulations, angiogenesis, cell growth, apoptosis, changes in basement membrane thickness, extracellular matrix organisation, MAPK signalling, are attributed to PKC isoforms. PKCepsilon and PKCδ have sometimes opposite effects. PKCepsilon is most important in stimulation of cell growth and differentiation and modulation of cytoskeleton These varied functions implicate PKC isoforms in cardiac hypertrophies and diabetic nephropathy and cardiovascular complications.
Protein kinase Cε (PKC epsilon) is expressed in low levels in several normal murine tissues with the exception of brain but is expressed at high levels in several hemopoietic cell lines and tumors, suggesting its possible role in tumorigenesis. Overexpression and stimulation of PKC ε causes an increased growth rate and higher cell densities in monolayers cultures.

Physical form

Rabbit Anti-Protein Kinase C ε is supplied as a liquid containing 0.1% sodium azide as preservative.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

3

Certificate of Analysis
Certificate of Origin
Isolation and characterization of PKC-L, a new member of the protein kinase C-related gene family specifically expressed in lung, skin, and heart.
Bacher NINA, et al.
Molecular and Cellular Biology, 11(1), 126-133 (1991)
Yoshiko Akita
The FEBS journal, 275(16), 3995-4004 (2008-07-19)
Recent studies have established essential roles of protein kinase Cepsilon in signaling pathways controlling various functions of microfilaments and intermediate filaments by modulating multiple cytoskeletal proteins. This review summarizes recent progress in our understanding of the roles of protein kinase...
Nicolas Duquesnes et al.
Journal of molecular and cellular cardiology, 51(5), 665-673 (2011-08-04)
Protein kinase C (PKC) is a family of 10 serine/threonine kinases divided into 3 subfamilies, classical, novel and atypical classes. Two PKC isozymes of the novel group, PKCε and PKCδ, have different and sometimes opposite effects. PKCε stimulates cell growth...
Differential roles of PKC alpha and PKC? in controlling the gene expression of Nox4 in human endothelial cells
Xu H, et al.
Free Radical Biology & Medicine, 44(8), 1656-1667 (2008)
Protein kinase C alpha promotes angiogenic activity of human endothelial cells via induction of vascular endothelial growth factor
Xu H, et al.
Cardiovascular Research, 78(2), 349-355 (2008)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.