P8651

Sigma-Aldrich

Protein A–Peroxidase from Staphylococcus aureus/horseradish

lyophilized powder

Synonym(s):
Peroxidase–Protein A
MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

form

lyophilized powder

Quality Level

application(s)

direct ELISA: 1:80,000 using human IgG

conjugate

peroxidase conjugate

storage temp.

−20°C

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General description

Protein A is a cell wall constituent of Staphylococcus aureus, with a molecular weight of 56kDa. It possesses five binding domains which interact with heavy chains of immunoglobulins at the Fc or Fab fragment. In response to protein A, the complement system, interferon production, hypersensitivity reactions and cell-mediated cytotoxicity get activated. Horseradish peroxidase is a heme-containing enzyme. In the presence of hydrogen peroxide, the enzyme catalyzes the oxidation of luminol.

Specificity

Binds IgG only from most mammals, except rat, goat, sheep.

Application

Protein A–Peroxidase from Staphylococcus aureus/horseradish has been used in enzyme-linked immunosorbent assay (ELISA).

Packaging

Package size based on protein content.

Physical form

Lyophilized powder containing sodium citrate buffer salt. Protein determined by A205.

Preparation Note

Protein A, extracellular, coupled to Peroxidase, Type VI, by a modification of the procedure of O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978), which favors low molecular weight conjugates.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

WGK Germany

3

Certificate of Analysis
Certificate of Origin
  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I prepare a stock solution of Product P8651, Protein A Peroxidase?

    A stock solution of this product can be prepared in PBS (phosphate buffered saline) or other neutral buffer at 1 mg/mL.

  4. What is the solution stability of Product P8651, Protein A Peroxidase?

    The solution should be stable for about one week when stored at 2-8°C. For long-term storage, the stock solution should be frozen in aliquots at -20 °C.

  5. How do I use Product P8651, Protein A Peroxidase, as a my secondary in Western blot analysis?

    For Western blotting, with chemiluminescent substrate, dilute 1:1000 to 1:10,000.

  6. I bought Product P8651, Protein A Peroxidase, to hide heavy and light chain in a Western blot. How does it do that?

    P8651 will only recognize the native antibody incubated on a blot, not what's run in the gel. This should circumvent heavy and light chain detection.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  10. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Identification of a Complex of the Three Forms of the Rat Liver
Asialoglycoprotein Receptor
James T. Sawyer
The Journal of Biological Chemistry, 263 (1998)
Gaetana Lanzi et al.
Journal of virology, 80(10), 4998-5009 (2006-04-28)
Deformed wing virus (DWV) of honeybees (Apis mellifera) is closely associated with characteristic wing deformities, abdominal bloating, paralysis, and rapid mortality of emerging adult bees. The virus was purified from diseased insects, and its genome was cloned and sequenced. The...
Horseradish peroxidase: a modern view of a classic enzyme
Nigel C
Phytochemistry, 65(3) (2004)
Florina Vlad et al.
The Plant cell, 21(10), 3170-3184 (2009-10-27)
The plant hormone abscisic acid (ABA) orchestrates plant adaptive responses to a variety of stresses, including drought. This signaling pathway is regulated by reversible protein phosphorylation, and genetic evidence demonstrated that several related protein phosphatases 2C (PP2Cs) are negative regulators...
Staphylococcal protein A consists of five IgG-binding domains
Tomas MOKS
European Journal of Biochemistry (1986)
Protocols
This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.
Read More
To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.
Read More

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