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P8787

Sigma-Aldrich

o-Phenylenediamine dihydrochloride

tablet, 4 mg substrate per tablet

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Synonym(s):
1,2-Phenylenediamine tablet, OPD, OPD Tablet
Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
Molecular Weight:
181.06
Beilstein/REAXYS Number:
3912045
MDL number:
PubChem Substance ID:
NACRES:
NA.83

Quality Level

form

tablet

mp

258 °C (dec.) (lit.)

storage temp.

2-8°C

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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1 of 4

This Item
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o-Phenylenediamine dihydrochloride tablet, 4 mg substrate per tablet

P8787

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 3 mg substrate per tablet

P8806

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet

P6912

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 1 mg substrate per tablet

P6662

o-Phenylenediamine dihydrochloride

form

tablet

form

tablet

form

tablet

form

tablet

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application

o-Phenylenediamine dihydrochloride has been used as a substrate for horseradish-peroxidase-conjugated secondary antibodies in enzyme-linked immunosorbent assay (ELISA).

Reconstitution

Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Carc. 2 - Muta. 2 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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