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P8811

Sigma-Aldrich

Protease from Streptomyces griseus

powder, BioReagent, suitable for mouse embryo cell culture, ≥3.5 units/mg solid

Synonym(s):
Actinase E, Pronase E
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.75

product line

BioReagent

Quality Level

form

powder

specific activity

≥3.5 units/mg solid

technique(s)

cell culture | embryo: suitable

impurities

starch, essentially free

storage temp.

−20°C

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This Item
P5147P065253702
specific activity

≥3.5 units/mg solid

specific activity

≥3.5 units/mg solid

specific activity

≥15 units/mg solid

specific activity

≥45,000 proteolytic units/g dry wt

technique(s)

cell culture | embryo: suitable

technique(s)

-

technique(s)

-

technique(s)

-

impurities

starch, essentially free

impurities

-

impurities

-

impurities

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

product line

BioReagent

product line

-

product line

-

product line

-

Specificity

A mixture of at least three proteolytic activities including an extracellular serine protease. In general, serine proteases display a wide range of substrate specificities, which are believed to be mediated by an active site composed of one Asp, one His, and a Ser residue in the molecule. This enzyme prefers to hydrolyze peptide bonds on the carboxyl side of glutamic or aspartic acid.

Application

Protease is typically used in nucleic acid isolation procedures in incubations of 0.5-3.0 hours supplemented with 0.2% sodium dodecyl sulfate and 10 mM EDTA.
This enzyme is more active at a higher pH range than the known alkaline protease, showing the proteolytic activity even in 0.2N NaOH solution. This enzyme is useful for proteolysis of insoluble protein and for structure investigation of protein.

Other Notes

An unusually non-specific protease.

Quality

Contains approximately 25% calcium acetate.

Physical properties

Completely inactivated by heating above 80 °C for 15-20 minutes.

Unit Definition

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

Preparation Note

Collected from culture broth of S. griseus.

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Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Shantha K Mahadevaiah et al.
Nature, 586(7830), 612-617 (2020-08-21)
Single-cell RNA sequencing of embryos can resolve the transcriptional landscape of development at unprecedented resolution. To date, single-cell RNA-sequencing studies of mammalian embryos have focused exclusively on eutherian species. Analysis of mammalian outgroups has the potential to identify deeply conserved
S Blumberg et al.
European journal of biochemistry, 136(1), 151-154 (1983-10-17)
A series of 2-mercaptoacetyl-dipeptides, a potential group of metalloendopeptidase inhibitors, has been synthesized by coupling the N-hydroxysuccinimide ester of S-acetyl-2-mercaptoacetic acid with hydrophobic dipeptide methyl ester hydrochlorides, followed by hydrolysis with NaOH in aqueous methanol and acidification with HCl. Thus
J Erik Mylroie et al.
Environmental toxicology and chemistry, 40(3), 780-791 (2020-10-13)
Perfluorooctanesulfonic acid (PFOS) is a perfluorinated compound used in many industrial and consumer products. It has been linked to a broad range of adverse effects in several species, including zebrafish (Danio rerio). The zebrafish embryo is a widely used vertebrate
Archana Vijaya Kumar et al.
Cancer science, 111(8), 2907-2922 (2020-06-24)
Heparan sulfate proteoglycans (HSPGs) act as signaling co-receptors by interaction of their sulfated glycosaminoglycan chains with numerous signaling molecules. In breast cancer, the function of heparan sulfate 2-O-sulfotransferase (HS2ST1), the enzyme mediating 2-O-sulfation of HS, is largely unknown. Hence, a
Yan Jiang et al.
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