Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse

clone PC 10, ascites fluid

Monoclonal Anti-Proliferating Cell Nuclear Antigen, Anti-PCNA

Quality Level

biological source


antibody form

ascites fluid

antibody product type

primary antibodies


PC 10, monoclonal


15 mM sodium azide

species reactivity

rat, mouse
yeast, monkey, insect, human


antibody small pack of 25 μL


flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:3,000 using human tonsil sections
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:3,000 using HS-68 human foreskin- cell extract





UniProt accession no.

shipped in

dry ice

storage temp.


Gene Information

human ... PCNA(5111)

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General description

PCNA (proliferating cell nuclear antigen) is a member of the structurally and functionally conserved family of DNA sliding clamps (β clamps). They exist as ring-shaped complexes- homotrimers in eukaryotes, having pseudohexameric symmetry. A monomer of PCNA is composed of two similar globular domains, connected by an interdomain connecting loop, which is a long, and probably a flexible loop. These monomers organize themselves in a head-to-tail manner to form a ring, which has an inner positively charged surface composed of α helices and an outer surface of β sheets.
Monoclonal Anti-Proliferating Cell Nuclear Antigen (mouse IgG2a isotype) is derived from the PC 10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with PCNA-Protein A fusion protein. Proliferating cell nuclear antigen (PCNA, 36 kDa), also known as cyclin, is an auxiliary protein of DNA polymerase. The protein is present in the nucleoplasm of continually cycling cells throughout the cell cycle.


Recognizes the acidic, non-histone, auxiliary protein of DNA polymerase, PCNA, also known as polymerase delta accessory protein. Fixation duration can markedly affect the intensity of PCNA immunoreactivity. However, delay in fixation does not affect the immunoreactivity. Enzymatic treatment destroys staining. In immunocytochemical labeling of acetone-methanol, or methanol-fixed cells, the antibody shows granular staining throughout the nucleus (nucleolus and nucleoplasm). Specific staining is observed in proliferating cell nuclei, particularly in germinal centers, of a wide range of normal and malignant tissues.


PCNA-Protein A fusion protein


Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse has been used in:
  • enzyme linked immuno sorbent assay (ELISA)
  • immunoblotting
  • immunohistology
  • immunoprecipitation
  • flow cytometry

Monoclonal Anti-Proliferating Cell Nuclear Antigen antibody produced in mouse has been used for immunohistochemistry (IHC) Western blotting (WB).

Biochem/physiol Actions

Proliferating cell nuclear antigen (PCNA) is essential for DNA replication during S-phase. It is essential for cellular DNA synthesis. PCNA is required for leading strand synthesis in the SV40 system where it acts as an auxiliary protein for polymerase.
PCNA (proliferating cell nuclear antigen) is loaded around the template-primer 3′ ends, which is recognized by the conserved chaperone-like complex RFC (replication factor C), and this mechanism is ATP-dependent. Encircling of the DNA by the PCNA ring ensures firm anchoring of the polymerases to the DNA, and hence, functions as a co-factor for DNA polymerases. Post-translation modifications of this protein at the K164 residue is required for the regulation of DNA damage tolerance (DDT) pathways, pathways that ensure recovery from replication arrest at DNA lesions.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Jan M J Aerts et al.
Fertility and sterility, 94(2), 708-714 (2009-05-09)
To develop and test a novel approach to xenotransplantation of isolated preantral follicles underneath the kidney capsule of immunodeficient mice. Prospective experimental animal study. Academic research unit. Healthy adult nude mice. Bovine ovaries from fetuses (n = 3) and calves...
Development of cerebellar neurons and glias revealed by in utero electroporation: Golgi-like labeling of cerebellar neurons and glias.
Kita Y et al
PLoS ONE, 8(7), e70091-e70091 (2013)
Relevance of simultaneous mono-ubiquitinations of multiple units of PCNA homo-trimers in DNA damage tolerance.
Kanao R et al
PLoS ONE, 10(2), e0118775-e0118775 (2015)
Proliferating cell nuclear antigen (PCNA): ringmaster of the genome
Paunesku T, et al.
International Journal of Radiation Biology, 77(10), 1007-1021 (2001)
L Hebbard et al.
Oncogene, 30(3), 301-312 (2010-09-08)
C-Src is infrequently mutated in human cancers but it mediates oncogenic signals of many activated growth factor receptors and thus remains a key target for cancer therapy. However, the broad function of Src in many cell types and processes requires...

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