The Poly-L-Lysine solution (P8920) was developed and tested to improve tissue adhesion to glass slides for histological and immunohistochemical use. Although other uses of P8920 have been reported, there is no data on hand regarding the use of P8920 to make the DNA binding stronger on polystyrene MaxiSorp plates.
P8920
Poly-L-lysine solution
0.1 % (w/v) in H2O
Synonym(s):
2-(4-Cyanoanilino)-4,6-dichloropyrimidine, 4-[(4,6-Dichloro-2-pyrimidinyl)amino]benzonitrile
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About This Item
Recommended Products
biological source
synthetic
shelf life
Expiry date on the label.
mol wt
150,000-300,000
concentration
0.1 % (w/v) in H2O
color
colorless
application(s)
hematology
histology
storage temp.
room temp
SMILES string
N([C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)C(=O)[C@@H](N)CCCCN
InChI
1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
InChI key
WBSCNDJQPKSPII-KKUMJFAQSA-N
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Application
Biochem/physiol Actions
Components
Preparation Note
comparable product
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Can I add this to maxisorp plates surfaces to make DNA binding stronger.
1 answer-
Helpful?
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Bonjour, Est ce que cette PLL est stérile ? Est-il possible de la filtrer ? Je vous remercie. Cordialement. Mme Boroni
1 answer-
Solutions of this product are not sterile and contain a preservative, ProClin, to control contamination. In theory you could sterile filter P8920 through low protein binding filters, such as PVDF or PES. Sterile filtering will not remove the ProClin from P8920. Live cells should not be exposed to ProClin, as ProClin interrupts the Krebs cycle at 4 different sites. It would be best to purchase the powder as P1524 and then sterile filter after the solution has been prepared.
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Hello! How many microscopy slides can I adhesive for every 100 milliliters of this solution( Poli-L- Lisine),? Gratitude!
1 answer-
The number of slides that may be coated is dependent upon the surface area. The general use instructions recommend using 0.5 - 1.0 mL per 25 cm2 coating area - the 25 cm2 value is based on a T-25 tissue culture flask. A standard microscope slide has an approximate area of 1.3 cm2.
Please see the link below to review the product datasheet which included a general protocol for coating: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/784/704/p6407pis.pdf
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What are the recommended uses for Product P8920, Poly-L-lysine solution?
1 answer-
P8920 was developed for use in coating slides intended for use in either histology or immunohistochemistry. Poly-L-lysine is preferred for uses such as silver staining procedures that require an alkaline pH. Silane coated slides may be more effective is a low pH is required.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product P8920, Poly-L-lysine solution, recommended for cell culture use?
1 answer-
No, the product contain 0.01% thimerosal as a preservative. For cell culture use, product P4832 is recommended. This solution has the same molecular weight as P8920 but contains no preservative. P4832 is sterile filtered and is considered suitable for cell culture use.
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