MilliporeSigma
All Photos(2)

P8999

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone DO-7, purified from hybridoma cell culture

Synonym(s):
Anti-Transformation-related protein 53, Anti-p53 antibody, Mouse monoclonal, Anti-TRP53
MDL number:
NACRES:
NA.41

Quality Level

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DO-7, monoclonal

form

buffered aqueous solution

mol wt

antigen ~53 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using human A431

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... TP53(7157)

General description

Monoclonal anti-p53, (mouse IgG2b isotype) is derived from the hybridoma DO-7 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on chromosome 17p. The p53 protein is highly conserved and expressed in normal tissues.

Application

Monoclonal Anti-p53 antibody produced in mouse has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunohistochemistry
  • immunoprecipitation

Biochem/physiol Actions

p53 is a transcription factor that regulates several critical cellular functions such as DNA repair, apoptosis, and cell cycle arrest. p53 primarily functions as a tumor suppressor and inactivates apoptosis inhibitors such as survivin, MCL-1 and BCL-2. Mutations in p53 gene occur most commonly in a wide range of human cancers. Thus, analysis of p53 function has important implications in cancer pathogenesis and therapy . Monoclonal Anti-p53 antibody specifically reacts with p53 in humans.
Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21waf1. p21waf1 (cyclin-dependent kinase inhibitor 1A) interacts directly with cyclin dependent kinases, important for cell cycle progression, thereby inhibiting their activity and resulting in cell cycle arrest.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Quotes and Ordering

Nalini Mehta et al.
Molecular therapy oncolytics, 4, 1-17 (2017-03-28)
Treatment of aggressive glioblastoma brain tumors is challenging, largely due to diffusion barriers preventing efficient drug dosing to tumors. To overcome these barriers, bacterial carriers that are actively motile and programmed to migrate and localize to tumor zones were designed.
Ting Zhang et al.
International journal of molecular medicine, 40(1), 21-30 (2017-05-13)
Dihydroartemisinin (DHA) has been shown to inhibit the viability of various cancer cells. Previous studies have revealed that the mechanisms involved in the inhibitory effects of DHA are based on theactivation of p53 and the mitochondrial-related cell death pathway. However
Role of p53, apoptosis, and cell proliferation in early stage Epstein-Barr virus positive and negative gastric carcinomas
Ishii HH, et al.
Journal of Clinical Pathology, 57(12), 1306-1311 (2004)
Chk2/hCds1 functions as a DNA damage checkpoint in G1 by stabilizing p53
Chehab NH, et al.
Genes & Development, 14(3), 278-288 (2000)
Norm D Smith et al.
The Journal of urology, 169(4), 1219-1228 (2003-03-12)
An extensive body of literature regarding p53 has accumulated during the last 2 decades. The cellular mechanisms of p53 are complex yet well-defined, whereas its clinical usefulness in the management of bladder cancer remains controversial. We outline the basic constitutive

Articles

p53: DNA Damage Response and Cancer

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

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