Skip to Content
MilliporeSigma
All Photos(2)

Documents

P8999

Sigma-Aldrich

Anti-p53 antibody, Mouse monoclonal

clone DO-7, purified from hybridoma cell culture

Synonym(s):

Anti-p53 antibody, Mouse monoclonal, Anti-TRP53, Anti-Transformation-related protein 53

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

DO-7, monoclonal

form

buffered aqueous solution

mol wt

antigen ~53 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.1-0.2 μg/mL using human A431

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TP53(7157)

Looking for similar products? Visit Product Comparison Guide

General description

Monoclonal anti-p53, (mouse IgG2b isotype) is derived from the hybridoma DO-7 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human wild type p53. The p53 gene is located on chromosome 17p. The p53 protein is highly conserved and expressed in normal tissues.

Application

Monoclonal Anti-p53 antibody produced in mouse has been used in:
  • immunoblotting
  • enzyme linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunohistochemistry
  • immunoprecipitation

Biochem/physiol Actions

Elevation of p53 protein induces the transcriptional activation of multiple genes, including p21waf1. p21waf1 (cyclin-dependent kinase inhibitor 1A) interacts directly with cyclin dependent kinases, important for cell cycle progression, thereby inhibiting their activity and resulting in cell cycle arrest.
p53 is a transcription factor that regulates several critical cellular functions such as DNA repair, apoptosis, and cell cycle arrest. p53 primarily functions as a tumor suppressor and inactivates apoptosis inhibitors such as survivin, MCL-1 and BCL-2. Mutations in p53 gene occur most commonly in a wide range of human cancers. Thus, analysis of p53 function has important implications in cancer pathogenesis and therapy . Monoclonal Anti-p53 antibody specifically reacts with p53 in humans.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Role of p53, apoptosis, and cell proliferation in early stage Epstein-Barr virus positive and negative gastric carcinomas
Ishii HH, et al.
Journal of Clinical Pathology, 57(12), 1306-1311 (2004)
Chk2/hCds1 functions as a DNA damage checkpoint in G1 by stabilizing p53
Chehab NH, et al.
Genes & Development, 14(3), 278-288 (2000)
Ting Zhang et al.
International journal of molecular medicine, 40(1), 21-30 (2017-05-13)
Dihydroartemisinin (DHA) has been shown to inhibit the viability of various cancer cells. Previous studies have revealed that the mechanisms involved in the inhibitory effects of DHA are based on theactivation of p53 and the mitochondrial-related cell death pathway. However
Norm D Smith et al.
The Journal of urology, 169(4), 1219-1228 (2003-03-12)
An extensive body of literature regarding p53 has accumulated during the last 2 decades. The cellular mechanisms of p53 are complex yet well-defined, whereas its clinical usefulness in the management of bladder cancer remains controversial. We outline the basic constitutive
Ruben Manuel Luciano Colunga Biancatelli et al.
Frontiers in physiology, 13, 812199-812199 (2022-04-08)
Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has caused more than 5 million deaths worldwide. Multiple reports indicate that the endothelium is involved during SARS-Cov-2-related disease (COVID-19). Indeed, COVID-19 patients display increased thrombophilia with arterial and venous embolism and lung microcapillary

Articles

p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service