P9029

o-Phenylenediamine

Peroxidase substrate, ≥98.0%, powder

• Synonym(s): 1,2-Diaminobenzene, 1,2-Phenylenediamine, OPD
• Empirical Formula (Hill Notation): C₆H₈N₂
• CAS Number: 95-54-5
• Molecular Weight: 108.14
• Beilstein: 606074
• EC Number: 202-430-6
• MDL number: MFCD00007721
• PubChem Substance ID: 24899004
• NACRES: NA.83

Properties

• vapor density: 3.7 (vs air)
• vapor pressure: 0.01 mmHg ( 25 °C)
• Assay: ≥98.0%
• form: powder
• color: white to off-white
• bp: 256-258 °C
• mp: 98-102 °C
• solubility: ethanol: 50 mg/mL, clear, colorless to faintly yellow
• fluorescence: λ_ex 337 nm; λ_em 417 nm (Pyruvic acid derivative)
• storage temp.: 2-8°C
• SMILES string: Nc1ccccc1N
• InChI: 1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2
• InChI key: GEYOCULIXLDCMW-UHFFFAOYSA-N

Description

General description

o-Phenylenediamine (OPD) Free Base is a peroxidase substrate suitable for use in enzyme linked immunosorbent assay (ELISA) procedures. The substrate produces a soluble product that is orange-brown in color and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H₂SO₄ and read at 492 nm.

Application

o-Phenylenediamine has been used in the microtiter?based assay for hyaluronan quantification. It has also been used in enzyme linked immunosorbent assay (ELISA) for color development.

Caution

May darken in storage.

Safety Information

• Pictograms: GHS06,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H301 - H312 + H332 - H317 - H319 - H341 - H351 - H410
• Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point(F): 276.8 °F - closed cup
• Flash Point(C): 136 °C - closed cup