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P9172

Sigma-Aldrich

Pyronin Y

for NA electrophoresis

Synonym(s):

Pyronin Y, Pyronin G, Pyronin J

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$261.00

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1 G
$70.10
5 G
$261.00

About This Item

Empirical Formula (Hill Notation):
C17H19ClN2O
CAS Number:
92-32-0
Molecular Weight:
302.80
Colour Index Number:
45005
Beilstein/REAXYS Number:
3795789
EC Number:
202-147-8
MDL number:
MFCD00011725
UNSPSC Code:
12171500
PubChem Substance ID:
57654569
NACRES:
NA.52

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grade

Molecular Biology

form

powder

composition

Dye content, ≥45%

technique(s)

nucleic acid detection: suitable

mp

250-260 °C (lit.)

solubility

water: 10 mg/mL, dark red

suitability

suitable for nucleic acid staining

storage temp.

room temp

SMILES string

[Cl-].CN(C)c1ccc2C=C3C=C\C(C=C3Oc2c1)=[N+](/C)C

InChI

1S/C17H19N2O.ClH/c1-18(2)14-7-5-12-9-13-6-8-15(19(3)4)11-17(13)20-16(12)10-14;/h5-11H,1-4H3;1H/q+1;/p-1

InChI key

INCIMLINXXICKS-UHFFFAOYSA-M

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1 of 4

This Item
21351983200P6892
Pyronin Y for NA electrophoresis

P9172

Pyronin Y

Pyronin Y certified by the BSC, Dye content 50 %

213519

Pyronin Y

Pyronin Y suitable for microscopy

83200

Pyronin Y

Pyronin B Dye content ≥30 %

P6892

Pyronin B

suitability

suitable for nucleic acid staining

suitability

-

suitability

-

suitability

-

technique(s)

nucleic acid detection: suitable

technique(s)

-

technique(s)

-

technique(s)

-

grade

for molecular biology

grade

certified by the Biological Stain Commission

grade

for microscopy (Bot., Fl., Hist.)

grade

-

solubility

water: 10 mg/mL, dark red

solubility

-

solubility

water: 0.01 g/10 mL, red

solubility

water: 1 mg/mL

form

powder

form

powder or crystals

form

-

form

powder

composition

Dye content, ≥45%

composition

Dye content, 50%

composition

-

composition

Dye content, ≥30%

Application

Pyronin Y has been used:
  • in the purification of quiescent and cycling hematopoietic stem cells and staining of bone marrow cells (HSCs)[1]
  • as a component of the polychlorinated biphenyls (PCBS) solution for staining mammary gland side population cells (MG-SPs)[2]
  • staining of CD34+ cells[3]

Biochem/physiol Actions

Pyronin Y is a cationic dye that intercalates RNA and is visible as a red band during electrophoresis. It has also been shown to accumulate in mitochondria of viable cells.[4] In conjuction with Methyl Green or Nancy-520, Pyronin Y can be used as a fluorescent differential stain of nucleic acids in paraffin tissue sections.

Preparation Note

This product is soluble in water 1 mg/ml, yielding a clear dark red solution.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000256525
SHBP6260
SHBP3367
SHBN0822
MKCB9857

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