MilliporeSigma
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P9279

Sigma-Aldrich

Phospholipase A2 from honey bee venom (Apis mellifera)

salt-free, lyophilized powder, 600-2400 units/mg protein

Synonym(s):
Lecithinase A, PLA2, Phosphatidylcholine 2-acylhydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.32

form

salt-free, lyophilized powder

Quality Level

specific activity

600-2400 units/mg protein

mol wt

14.5 kDa

UniProt accession no.

storage temp.

−20°C

Gene Information

honey bee ... Pla2(406141)

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General description

Phospholipase A2 from honey bee venom (Apis mellifera) is a calcium-dependent lipolytic enzyme.

Application

Phospholipase A2 from honey bee venom (Apis mellifera) has been used
  • to determine renal proximal tubular segments (PTS) viability during oxygenation and hypoxia-reoxygenation
  • in the treatment of photosystem-II (PSII) dimer complex of Synechocystis to study changes in oxygen-evolving activity
  • as a standard in phospholipase activity assay

Packaging

1, 5, 25 mg in glass bottle

Biochem/physiol Actions

Hydrolyzes the β-ester bond of zwitterionic glycerophospholipids. Preferred substrates are phosphatidylcholine, phosphatidylethanolamine, and their plasmalogen analogues. Phosphatidylinositol and phosphatidylserine are also hydrolyzed. Aggressively attacks phospholipids in membranes of intact cells.

Unit Definition

One unit will hydrolyze 1.0 μmole of soybean L-α-phosphatidylcholine to L-α-lysophosphatidylcholine and a fatty acid per min at pH 8.9 at 25 °C.

Analysis Note

Protein determined by biuret.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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