Please consult the User Guide for all procedures recommended while using this product. If the information is not in the User Guide, we have not tested other conditions out. Link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/306/054/p9424pis-ms.pdf
P9424
ProteinA-Sepharose™ 4, Fast Flow from Staphylococcus aureus
aqueous ethanol suspension
Synonym(s):
Protein A–Agarose
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 mL | Available to ship TODAYfromMILWAUKEE | $359.00 | |
| 5 mL | Available to ship TODAYfromMILWAUKEE | $1,190.00 |
About This Item
Form:
aqueous ethanol suspension
Biological source:
Staphylococcus aureus
$359.00
Available to ship TODAYDetails
biological source
Staphylococcus aureus
Quality Level
form
aqueous ethanol suspension
analyte chemical class(es)
proteins (Immunoglobulins of various mammalian species)
extent of labeling
~6 mg per mL
technique(s)
affinity chromatography: suitable
matrix
Sepharose 4B Fast Flow
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
capacity
≥30 mg/mL binding capacity (human IgG)
storage temp.
2-8°C
General description
Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.[1]
Application
Protein A-Sepharose is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Physical form
Suspension in 20% ethanol
Legal Information
Sepharose is a trademark of Cytiva
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This Item | |||
|---|---|---|---|
| technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable | technique(s) affinity chromatography: suitable | technique(s) - |
| biological source Staphylococcus aureus | biological source - | biological source - | biological source - |
| form aqueous ethanol suspension | form lyophilized powder | form aqueous ethanol suspension | form cell suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| matrix Sepharose 4B Fast Flow | matrix Sepharose CL-4B | matrix Sepharose 4B Fast Flow | matrix - |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
wgk
WGK 3
Storage Class
3 - Flammable liquids
flash_point_f
104.0 °F
flash_point_c
40 °C
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| P9424-1ML | 04061834403111 |
| P9424-5ML | 04061838065223 |
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For ChIP-seq experiments, what is the correct procedure to prepare a 50% Protein A–Sepharose bead slurry? Specifically, can the slurry be prepared by pelleting 20 µL of the stock beads at 1,000 g for 30 s, discarding the storage buffer, and resuspending
1 answer-
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