Documents

P9489

Sigma-Aldrich

Anti-p35 (Cdk5 Regulator) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

MDL number:
NACRES:
NA.46
Pricing and availability is not currently available.

biological source

rabbit

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 35 kDa

species reactivity

human, rat

application(s)

microarray: suitable
western blot: 1:1,000 using a rat brain extract (cytosolic fraction)

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CDK5R1(8851)
rat ... Cdk5r1(116671)

General description

Cell division protein kinase 5 (Cdk5) is a serine/threonine kinase and shares homology to other Cdks. It belongs to cdk family.

Immunogen

synthetic peptide corresponding to the C-terminus of p35 (Cdk5R), (human, amino acids 192-209).

Application

Anti-p35 (Cdk5 Regulator) antibody produced in rabbit has been used in western blotting and immunohistochemistry.

Biochem/physiol Actions

Cell division protein kinase 5 (Cdk5) kinase activity can be detected mainly in postmitotic neurons in the central nervous system (CNS). Cdk5/p35 activity is required in the mature CNS. Cdk5 phosphorylates dopamine and cyclic adenosine 3′,5′-monophosphate-regulated phosphoprotein of 32 kDa (DARPP-32) and Syntaxin-binding protein 1 (Munc18), which in turn affects synaptic vesicle endocytosis. Deregulation of Cdk5 activity contributes to the pathogenesis of neurodegenerative diseases such as Alzheimer′s disease (AD). It mediates neuronal migration during cortical development. Cdk5-/- mice exhibit embryonic lethality associated with disruption of the cortical laminar structures in the cerebral cortex, olfactory bulb, hippocampus, and cerebellar cortex. Mice lacking p35 display defects in cortical lamination and fasciculation of axonal fibers. In developing mice brain, Cdk5/p35 interacts with Reelin/Dab1 and positions of cortical neurons.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

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Certificate of Analysis
Certificate of Origin
Cyclin-dependent kinase 5/p35 contributes synergistically with Reelin/Dab1 to the positioning of facial branchiomotor and inferior olive neurons in the developing mouse hindbrain
Ohshima T, et al.
Neuroscience journal, 22(10), 4036-4044 (2002)
Activation of cyclin-dependent kinase 5 is a consequence of cell death
Ye Y, et al.
BioMed Research International, 2009 (2009)
Deregulated Cdk5 activity is involved in inducing Alzheimer?s disease
Shukla V, et al.
Archives of Medical Research, 43(8), 655-662 (2012)
Cyclin-dependent kinase 5 associated with p39 promotes Munc18-1 phosphorylation and Ca2+-dependent exocytosis
Lilja L, et al.
The Journal of Biological Chemistry, 279(28), 29534-29541 (2004)
NGF controls APP cleavage by downregulating APP phosphorylation at Thr668: relevance for Alzheimer's disease
Triaca V, et al.
Aging Cell, 15(4), 661-672 (2016)
Articles
Alzheimer's disease (AD) is the most common cause of dementia in the elderly and is characterized by gradual loss of cognitive functions.
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