MilliporeSigma
All Photos(1)

P9620

Sigma-Aldrich

Puromycin dihydrochloride

Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, suitable for cell culture

Synonym(s):
3′-(L-α-Amino-p-methoxyhydrocinnamamido)-3′-deoxy-N,N-dimethyladenosine dihydrochloride, Stylomycin dihydrochloride
CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.85

biological source

Streptomyces alboniger

Quality Level

type

for cell culture

Assay

>98% (HPLC)

form

solution

concentration

10 mg/mL in H2O

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 10 mg/mL

antibiotic activity spectrum

Gram-positive bacteria
neoplastics
parasites

Mode of action

protein synthesis | interferes

shipped in

wet ice

storage temp.

−20°C

SMILES string

Cl.Cl.COc1ccc(C[C@H](N)C(=O)N[C@H]2[C@@H](O)[C@@H](O[C@@H]2CO)n3cnc4c(ncnc34)N(C)C)cc1

InChI

1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1

InChI key

MKSVFGKWZLUTTO-FZFAUISWSA-N

Related Categories

Compare Similar Items

View Full Comparison

1 of 4

This Item
A9415SBR00001L2774
Actinomycin D from Streptomyces sp., suitable for cell culture, ≥95%

Sigma-Aldrich

A9415

Actinomycin D

biological source

Streptomyces alboniger

biological source

Streptomyces sp.

biological source

Streptomyces orientalis

biological source

-

assay

>98% (HPLC)

assay

≥95%

assay

-

assay

-

form

solution

form

powder

form

liquid

form

-

concentration

10 mg/mL in H2O

concentration

-

concentration

100 mg/mL in DMSO

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

solubility

H2O: soluble 10 mg/mL

solubility

ethanol, DMSO: soluble (Stable in aqueous solutions at 2-8 °C.)

solubility

-

solubility

H2O: 4 mL, clear, colorless to faintly yellow (200 mg + 4 mL H2O)

General description

Chemical structure: peptidyl nucleoside

Application

Puromycin is an aminonucleoside antibiotic that is derived from Streptomyces alboniger. It allows selection for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). It has been used to study vascular smooth muscle cell viability after treatment in the rabbit model . Puromycin is used to produce enhanced green fluorescent protein (EGFP) transgenic piglets after somatic cell cloning and embryo transfer.

Biochem/physiol Actions

Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3′-terminal end of the aminoacyl-tRNA. Puromycin also acts as a reversible inhibitor of dipeptidyl-peptidase II (serine peptidase) and cytosol alanyl aminopeptidase.

Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.

Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.

Preparation Note

This product is concentrated at 10 mg/mL in water. It has been tested on HeLa cells for cell growth arrest and selection of cells after transfection of the pac resistance gene.

Other Notes

Keep container tightly closed in a dry and well-ventilated place.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

(enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service