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MilliporeSigma

P9740

PHOS-Select™ Iron Affinity Gel

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UNSPSC Code:
12352200
NACRES:
NA.32

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form

suspension

shelf life

2 yr at −20‑0 °C

pH

~5

capacity

≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C)

storage temp.

−20°C

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This Item
H8162H0537I1408
PHOS-Select™ Iron Affinity Gel

Sigma-Aldrich

P9740

PHOS-Select™ Iron Affinity Gel

HIS-Select® Cobalt Affinity Gel (1:1 suspension in a 30% ethanol solution)

Millipore

H8162

HIS-Select® Cobalt Affinity Gel

HIS-Select® HF Nickel Affinity Gel

Millipore

H0537

HIS-Select® HF Nickel Affinity Gel

IMAC-Select Affinity Gel (1:1 suspension in a 30% ethanol solution)

Sigma-Aldrich

I1408

IMAC-Select Affinity Gel

form

suspension

form

(1:1 suspension in a 30% ethanol solution)

form

suspension

form

(1:1 suspension in a 30% ethanol solution)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shelf life

2 yr at −20‑0 °C

shelf life

1 yr (when stored properly.)

shelf life

-

shelf life

1 yr

pH

~5

pH

-

pH

-

pH

-

capacity

≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C)

capacity

>15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein)

capacity

15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)

capacity

10-30 μmol/mL binding capacity (Cu+2)

General description

The PHOS-Select™ iron affinity gel is a novel iron [Fe(III)] chelate matrix. This matrix provides high capacity affinity binding of molecules containing phosphate groups.

Application

Phos-Select™ products facilitate a number of applications including:
  • affinity enrichment of phosphopeptides obtained from protein tryptic digests
  • direct transfer of phosphocompounds for analysis (HPLC or mass spectrometry)
  • enrichment of phosphopeptides by immobilized metal affinity chromatography (IMAC)[1][2]

Physical form

aqueous glycerol suspension

Other Notes

Phos-Select™ Iron Affinity Gel is a novel iron [Fe(III)] chelate matrix based on Sigma′s NTA analog chelate ligand. The matrix provides high capacity affinity binding of molecules containing phosphate groups.

Legal Information

PHOS-Select is a trademark of Sigma-Aldrich Co. LLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000354485
0000354485
SLBR7938V
SLBD8559V
SLBB2844V

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Jie Gao et al.
Scientific reports, 7, 40852-40852 (2017-01-21)
Low temperature is one of the key environmental stresses, which greatly affects global banana production. However, little is known about the global phosphoproteomes in Musa spp. and their regulatory roles in response to cold stress. In this study, we conducted
The SCX/IMAC enrichment approach for global phosphorylation analysis by mass spectrometry
Villen J and Gygi SP
Nature Protocols, 3(10), 1630-1630 (2008)
Quantitative Phosphoproteomics of the ATM and ATR dependent DNA damage response in Arabidopsis thaliana
Roitinger E, et al.
Molecular and Cellular Proteomics, 3(10), mcp-M114 (2015)
Jason A Wojcechowskyj et al.
PloS one, 6(9), e24918-e24918 (2011-09-29)
CXCL12 (SDF-1) is a chemokine that binds to and signals through the seven transmembrane receptor CXCR4. The CXCL12/CXCR4 signaling axis has been implicated in both cancer metastases and human immunodeficiency virus type 1 (HIV-1) infection and a more complete understanding
Mathilde Biola-Clier et al.
Nanomaterials (Basel, Switzerland), 10(2) (2020-01-25)
TiO2 nanoparticles (NPs) are one of the most produced NPs worldwide and are used in many consumer products. Their impact on human health, especially through inhalation, has been studied for more than two decades. TiO2 is known for its strong
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