PCG2001

Sigma-Aldrich

TruPAGE Precast Gels

10%, 10 x 10cm, 12-well

Synonym(s):
Precast Polyacrylamide Gels, Precast Gels for SDS-PAGE
NACRES:
NB.22

shelf life

24 mo. at 2‑8 °C (from the date of manufacture)

packaging

box of 10 ea

concentration

10% polyacrylamide

cassette W × L

10 cm × 10 cm

gel thickness

1 mm

well size × volume

12 wells × 35 μL

compatibility

for use with Life Technologies XCell SureLock® XCell I, XCell II Mini-Cell and Bolt® Mini Gel Tank
for use with Lonza Ltd PAGEr Minigel Chamber

storage temp.

2-8°C

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Application

TruPAGE Precast Gels is suitable for use for the electrophoretic separation of hepatocytes protein lysate and breast cancer MDA-MB-231 cell lysate prior to western blot analysis.

Features and Benefits

TruPAGE SDS-PAGE Precast Gels provide improved protein resolution, tear resistance, and an extended shelf-life. TruPAGE gels are formulated with Triethanolamine (TEA)-Tricine, featuring a neutral pH running environment that helps prevents protein modification during the course of the experiment.
The gels come in two gel cassette sizes (10 x 10 cm and 10 x 8 cm) for increased equipment compatibility. TruPAGE gels have extra tall wells to prevent lane-to-lane overflow and come in two well formats (12-well and 17-well). TruPAGE gels are available in several different gel concentrations (10%, 12%, 4-8%, 4-12% and 4-20%) to provide desired resolution of proteins of any size. Please refer to the migration chart to select the appropriate precast gel and running buffer combination for your experimental needs. Only use TruPAGE formulated buffers when running TruPAGE gels to ensure optimal results and reproducibility. Please review the
TruPAGE Technical Bulletin for buffer formulation recipes and general running guidelines.

  • Long Shelf Life - 2 years from the date of manufacture when stored properly.
  • High Performance - Extended full length resoling gels provides improved resolution.
  • Durable - Stronger than standard polyacrylamide gels, preventing tearing during post-electrophoresis processing.
  • Extra Tall Wells - Eliminate sample spill over and accommodate larger sample loading volumes with well teeth that protrude above the back plate of the gel cassette.
  • Excellent Compatibility - TruPAGE gels are compatible with the popular gel running equipment, including Sigma-Aldrich′s Dual Run Tank (Z741768) and other manufacturers like Life Technologies, Bio-Rad®, Lonza®, Hoefer®, CBS Scientific as well as others, allowing you to continue using the equipment you already own.
  • Fast - Run times as low as 30 minutes.
  • Great Value - Gels and buffers offer a cost-saving alternative without sacrificing performance and experimental integrity.

TruPAGE Precast Gels Migration Chart

Legal Information

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
Bolt is a registered trademark of Life Technologies
Hoefer is a registered trademark of Hoefer, Inc.
Lonza is a registered trademark of Lonza Group Ltd.
PAGEr is a trademark of Lonza Group Ltd.
TruPAGE is a trademark of Sigma-Aldrich Co. LLC
XCell I is a trademark of Life Technologies
XCell II is a trademark of Life Technologies
XCell SureLock is a registered trademark of Life Technologies

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Vasoactive intestinal peptide induces proliferation of human hepatocytes
Khedr MEMS, et al.
Cell proliferation, 51(5), e12482-e12482 (2018)
Overexpression of ROD1 inhibits invasion of breast cancer cells by suppressing the translocation of beta-catenin into the nucleus
Zhou Y, et al.
Oncology Letters, 16(2), 2645-2653 (2018)
Yousef Risha et al.
Scientific reports, 10(1), 13572-13572 (2020-08-13)
Cancer cells release small extracellular vesicles, exosomes, that have been shown to contribute to various aspects of cancer development and progression. Differential analysis of exosomal proteomes from cancerous and non-tumorigenic breast cell lines can provide valuable information related to breast...
Wen-Chen Ji et al.
Neurological research, 42(5), 361-371 (2020-03-10)
Objectives: Spinal cord injury (SCI) is a most debilitating traumatic injury, and cytotherapy is a promising alternative treatment strategy. Here we investigated the effect and mechanism of adipose-derived stem/stromal cells (ASCs) with overexpressing brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT3)...
Zili Zhang et al.
Autophagy, 16(8), 1482-1505 (2019-11-05)
Ferroptosis is a recently discovered form of programmed cell death, but its regulatory mechanisms remain poorly understood. Here, we show that the RNA-binding protein ZFP36/TTP (ZFP36 ring finger protein) plays a crucial role in regulating ferroptosis in hepatic stellate cells...

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