TruPAGE Precast Gels

4-20%, 10 x 8cm, 12-well

Precast Polyacrylamide Gels, Precast Gels for SDS-PAGE

shelf life

24 mo. at 2‑8 °C (from the date of manufacture)


box of 10 ea


4-20% polyacrylamide

cassette W × L

10 cm × 8 cm

gel thickness

1 mm

well size × volume

12 wells × 35 μL


for use with Bio-Rad Mini-PROTEAN® Tetra Cell, Mini-PROTEAN Dodeca, Mini-PROTEAN II and 3 cells (reverse device gasket to flat side)
for use with Hoefer Mighty Small II and Tall Mighty Small (SE 260 and SE280)

storage temp.


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Features and Benefits

TruPAGE Precast Gels 4-20% have been used to separate proteins through sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by Western blotting.
TruPAGE SDS-PAGE Precast Gels provide improved protein resolution, tear resistance, and an extended shelf-life. TruPAGE gels are formulated with Triethanolamine (TEA)-Tricine, featuring a neutral pH running environment that helps prevents protein modification during the course of the experiment.
The precast gels for SDS-PAGE come in two gel cassette sizes (10 x 10 cm and 10 x 8 cm) for increased equipment compatibility. TruPAGE gels have extra tall wells to prevent lane-to-lane overflow and come in two well formats (12-well and 17-well). TruPAGE gels are available in several different gel concentrations (10%, 12%, 4-8%, 4-12% and 4-20%) to provide desired resolution of proteins of any size. Please refer to the migration chart to select the appropriate precast gel and running buffer combination for your experimental needs. Only use TruPAGE formulated buffers when running TruPAGE gels to ensure optimal results and reproducibility. Please review the
TruPAGE Technical Bulletin for buffer formulation recipes and general running guidelines.

  • Long Shelf Life - 2 years from the date of manufacture when stored properly.
  • High Performance - Extended full length resoling gels provides improved resolution.
  • Durable - Stronger than standard polyacrylamide gels, preventing tearing during post-electrophoresis processing.
  • Extra Tall Wells - Eliminate sample spill over and accommodate larger sample loading volumes with well teeth that protrude above the back plate of the gel cassette.
  • Excellent Compatibility - TruPAGE gels are compatible with the popular gel running equipment, including Sigma-Aldrich′s Dual Run Tank (Z741768) and other manufacturers like Life Technologies, Bio-Rad®, Lonza®, Hoefer®, CBS Scientific as well as others, allowing you to continue using the equipment you already own.
  • Fast - Run times as low as 30 minutes.
  • Great Value - Gels and buffers offer a cost-saving alternative without sacrificing performance and experimental integrity.
TruPAGE Precast Gels Migration Chart
There is no tape to remove. There is also no comb to remove, which prevents torn or bent wells. Instead, the cassettes lock well fingers in place.

Legal Information

Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
Dodeca is a trademark of Bio-Rad Laboratories, Inc.
Hoefer is a registered trademark of Hoefer, Inc.
Lonza is a registered trademark of Lonza Group Ltd.
Mini-PROTEAN is a registered trademark of Bio-Rad Laboratories, Inc.
TruPAGE is a trademark of Sigma-Aldrich Co. LLC


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Registered report: Transcriptional amplification in tumor cells with elevated c-Myc.
Blum D, et al.
eLife, 4, e04024-e04024 (2015)
Quick and facile preparation of histone proteins from the green microalga Chlamydomonas reinhardtii and other photosynthetic organisms.
Wong, et al.
Methods, 184, 102-111 (2020)
Camila Yattah et al.
Neurochemical research, 45(3), 606-619 (2020-02-06)
Differentiation of oligodendrocytes (OL) from progenitor cells (OPC) is the result of a unique program of gene expression, which is further regulated by the formation of topological domains of association with the nuclear lamina. In this study, we show that...

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